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Title:: Influences of endothelin1 on apoptosis of hypoxia/reoxygenation myocardiocytes

作者:: 郭万刚¹ ; 余志斌¹; 孙立军²; 罗中华²; 第四军医大学：1.航空航天医学系航空航天生理学教研室，2.西京医院放射科，陕西西安 710032

Author(s):: GUO Wangang¹; YU Zhibin¹; SUN Lijun²; LUO Zhonghua²; 1.Department of Aerospace Physiology, School of Aerospace Medicine, 2.Departmdnt of Radiology,Xijing Hospital,Fourth Military Medical University, Xi′an 710032, Shaanxi, China

关键词:: 心肌细胞; 缺氧/复氧; 细胞凋亡; 内皮素1

Keywords:: cardiomyocytes; hypoxia/reoxyegenation; apoptosis; endothelin

分类号:: Q255;R331.36

DOI:: -

文献标识码:: A

摘要:: 目的探讨在缺氧/复氧过程中不同时期给予内皮素1（ET1）对缺氧/复氧所致心肌损伤与凋亡的影响。方法乳鼠心肌细胞缺氧/复氧模型，流式细胞仪检测心肌细胞凋亡，Hoechst 33258染色计算凋亡率，试剂盒检测乳酸脱氢酶（LDH）的活性。结果 ET1预处理组（EP）细胞生存率较缺氧复氧组(HR)提高，凋亡率下降，LDH活性下降；ET1缺氧即刻处理组(EH)细胞生存率较HR组降低，凋亡率升高，LDH释放量增加；ET1复氧处理组(ER)的细胞生存率、凋亡率及LDH活性与HR组均无统计学差异。结论 ET1预处理有心肌细胞保护作用，ET1缺氧时有促进心肌细胞损伤和凋亡的作用。

Abstract:: AIM To explore the effects of endothelin1 at different administrative timing on apoptosis and cell injury induced by hypoxia/reoxygenation. METHODS Primarily cultured neonatal rat cardiomyctes were treated with 950 ml/L N2-50 ml/L CO2 mixture to establish hypoxia/reoxygenation model. Flow cytometry and Hoechst 33258 stain were performed to determine the apoptotic rates of cardiomyocytes. A domestic kit was used to determine the activity of lactate dehydrogenase (LDH). RESULTS Cell viability values, apoptotic rates and lactate dehydrogenase (LDH) activity of cells with 24 h ET1 treatment before hypoxia increased, lowered and decreased respectively compared with those in hypoxia/reoxygenation group (HR). ET1 treatment during hypoxia depressed the cell viability, raised apoptotic rate and LDH release. ET1 treatment during reoxygenation had no effects on these values. CONCLUSIONET1 pretreatment has protective effects on cardiomyocytes from hypoxia/reoxygenation. However, ET1 during hypoxia aggravates the injury of cardiomyocytes induced by hypoxia/reoxygenation.

参考文献/References

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内皮素1对缺氧/复氧所致心肌细胞凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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