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Title:: Sympathetic neural remodeling after myocardial infarction correlates with growth associated protein43 in rabbits

作者:: 张超; 郑强荪; 高渊; 雷靖祎; 刘文秀; 张中; 张宪伟; 第四军医大学唐都医院心内科,陕西西安 710038

Author(s):: ZHANG Chao; ZHENG Qiangsun; GAO Yuan; LEI Jingyi; LIU Wenxiu; ZHANG Zhong; ZHANG Xianwei; Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038, Shaanxi, China

关键词:: 心肌梗塞; 交感神经; 神经重构; 生长相关蛋白; β受体阻滞剂

Keywords:: myocardial infarction; sympathetic nerve; neural remodeling; growth associated protein43; βblocker

分类号:: R541.4

DOI:: -

文献标识码:: A

摘要:: 目的探讨家兔心肌梗死(MI)后生长相关蛋白(growth associated protein ,GAP)43的动态表达及其与交感神经重构的关系，并探讨β受体阻滞剂在MI后交感神经重构中的作用。方法从MI家兔的梗死周边、室间隔和右心室取材，通过免疫组化染色并结合计算机图像处理技术检测MI后3、7、30 d及普奈洛尔干预后GAP43的表达及交感神经支配的密度和范围。结果 MI后3 d，梗死周边、室间隔以及右心室中GAP43表达显著增加（P<0.01）。梗死周边以及室间隔心肌中交感神经支配密度显著降低(P<0.01)，右心室中交感神经支配密度显著增加(P<0.01), 右心室中交感神经支配与GAP43的表达相关(P<0.05)；MI后7 d, 梗死周边、室间隔以及右心室中GAP43表达以及交感神经支配密度均显著增加(P<0.01), 梗死周边心肌中GAP43的表达与交感神经支配密度相关(P<0.01)；MI后30 d，梗死周边、室间隔以及右心室交感神经支配密度有继续增加的趋势。梗死周边、室间隔以及右心室中GAP43表达显著降低(P<0.01)。普奈洛尔促使MI后各部位交感神经支配的范围趋于正常化，并降低室间隔和右心室中的交感神经支配密度(P<0.05)。结论 MI后GAP43的表达具有动态过程，并与交感神经支配密度的变化相关。β受体阻滞剂能改善MI后的交感神经重构。

Abstract:: AIM To explore the expression of growth associated protein43 (GAP43) and its effect on neural remodeling after myocardial infarction (MI) in rabbits and the effect of βblocker on neural remodeling. METHODS Samples were obtained from the MI rabbits in periinfarcted area, ventricular septum and right ventricle (RV). By immunohistochemistric method and computer image processing technique, the expression of GAP43 and the density and extent of sympathetic innervation were examined in different groups (3 days, 7 days, 30 days after MI and propranolol taken orally). RESULTS In 3 day group, the expressions of GAP43 were significantly higher in periinfarcted area and higher in RV and ventricular septum than those in control areas (P<0.01), the density of sympathetic nerve decreased in periinfarcted area and ventricular septum (P<0.01) and increased in RV significantly(P<0.01), and GAP43 expression correlated with sympathetic innervation in RV (P<0.05). In 7 day group, the expressions of GAP43 and density of sympathetic nerves in periinfarcted area, ventricular septum and RV significantly increased (P<0.01), and GAP43 expression correlated with sympathetic innervation in periinfarcted area (P<0.01). In 30 day group, the expressions of GAP43 decreased significantly in periinfarcted area, ventricular septum and RV (P<0.01), and the density and the extent of sympathetic nerve were higher than those in 3 and 7 day groups. Propranolol decreased the density of sympathetic innervation in ventricular septum and RV (P<0.05) and normalized the extent of sympathetic innervation in the whole ventricle after left ventricular MI. CONCLUSION The expression of GAP43 in myocardium is dynamic and correlated with the density and extent of sympathetic innervation. βblocker plays an important role in improving this sympathetic neural remodeling.

参考文献/References

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