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Title:: Homing ability of mesechymal stem cells to damaged myocardium of doxorubicininduced heart failure rats by vein transplantation

作者:: 徐瑾; 王彬尧; 陈颖敏; 何奔; 上海交通大学医学院附属仁济医院心内科，上海 200001

Author(s):: XU Jin; WANG Binyao; CHEN Yingmin; HE Ben; Department of Cardiology, Renji Hospital, Shanghai Jiaotong University, Shanghai 200001, China

关键词:: 骨髓间充质干细胞; 细胞移植; 心力衰竭

Keywords:: mesechymal stem cells; cell transplantation; heart failure

分类号:: R541.61；R459.9

DOI:: -

文献标识码:: A

摘要:: 目的采用2脱氧5氮杂胞苷（5aza2’deoxycytidine，5azaCdR）诱导的骨髓间充质干细胞（mesechymal stem cells, MSCs）经尾静脉移植入阿霉素心肌病心衰大鼠中，探讨其向病损心肌趋化情况。方法体外培养Wistar大鼠的MSCs，用0.3 μmol/L的5azaCdR体外两次诱导第2代MSCs，然后将其用溴氮胞苷（bromodeoxyuridine，BrdU）标记后经尾静脉植入阿霉素造模成全心衰心肌病的大鼠，同时以注射无血清培养基(DMEM)的实验动物为对照组。移植后3 d和1月，分别处死部分大鼠，通过免疫组化研究MSCs向病损心肌的趋化情况及其在心肌局部分布特点。结果移植后3 d，MSCs移植组大鼠心肌中发现BrdU(+)细胞，左室心肌中分布最多，右心室也有少量分布；并且阳性细胞与自身心肌细胞均匀分布。移植后1月，MSCs仍能在心肌中存活，沿心肌纤维方向排列，并且具有心肌细胞的核像，呈长梭形。结论经5azaCdR诱导的MSCs经尾静脉移植入阿霉素心肌病心衰大鼠，其能向心脏靶向性迁徙；可存活达1月并具有部分心肌细胞特点。

Abstract:: AIM To investigate the homing ability of 5aza2′deoxycytidine (5azaCdR) induced mesechymal stem cells (MSCs) to damaged myocardium of rats with doxorubicininduced heart failure by tail vein transplantation. METHODS MSCs of Wistar rats were cultured in vitro and the second passage MSCs were incubated together with 5azaCdR (0.3 μmol/L) for two times. The induced MSCs labeled with bromodeoxyuridine (BrdU) were then transplanted through tail vein into the rats in the model of global heart failure induced by doxorubicin application. The rats receiving serumfree medium injection were used as controls. Three days and 1 month after transplantation, the homing ability of MSCs to damaged myocardium and the distributing characteristics were studied by immunohistochemistry. RESULTS Three days after transplantation in the model of doxorubicininduced dilated cardiomyopathy, the donor antiBrdU positive cells were found in rats′ myocardium, predominant in the left ventricle. The donor MSCs distributed well mixing with rats′ cardiomyocytes. One month after transplantation, donor MSCs were found to have arranged with myocardial fibers and formed cardiomyocytelike structure such as spindleshaped nucleus. CONCLUSION The MSCs have the homing ability to the myocardium of rats with doxorubicininduced dilated cardiomyopathy through tail vein and can survive more than 1 month in the myocardium with some features of cardialmyocytes.

参考文献/References

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经静脉移植骨髓间充质干细胞向扩张性心肌病大鼠心肌趋化的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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