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|本期目录/Table of Contents|

硫化氢改良的St.Thomas Ⅱ液保存离体大鼠心脏不同保存时限超微结构的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
531-534
栏目:
基础研究
出版日期:
2007-10-01

文章信息/Info

Title:
Ultrastructural change of isolated rat hearts after hypothermic preservation by St.Thomas Ⅱ solutions containing H2S
作者:
白崇峰; 崔勤; 李亮; 李彤
第四军医大学西京医院心血管外科，陕西 西安 710032
Author(s):
BAI Chongfeng;  CUI Qin;  LI Liang;  LI Tong
Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi,China
关键词:
硫化氢; KATP通道; 心脏移植; 器官保存; 超微结构
Keywords:
Hydrogen sulfide;  KATP channel;  Heart transplantation;  Organ preservation;  Ultrastructure
分类号:
R541
DOI:
-
文献标识码:
A
摘要:
目的 探讨含硫化氢（H2S）的St.Thomas Ⅱ液，对离体大鼠心脏低温下不同保存时间再灌注30 min后，心肌超微结构的变化。方法 SD大鼠32只随机分成4组，每组8只，分别为：低温保存4 h组，低温保存6 h组，低温保存8 h组，低温保存10 h组。停搏与保存均用含硫化氢（H2S）的St.Thomas Ⅱ液进行低温保存。采用Langendorff心脏灌注和工作模型装置，进行心脏保存前及保存后再灌注30 min心功能测定，比较心功能恢复率、心肌酶（CK，LDH）漏出量，以及超微结构心肌组织变化。结果 随着保存时间的延长心功能恢复率逐渐降低，心肌酶漏出量逐渐增加。在保存4 h和6 h后心肌细胞出现轻度损伤，在保存8 h和10 h后，心肌细胞呈现中度损伤，但保存10 h后的离体心脏再灌注后心功能的恢复率与保存8 h后相比较存在显著的差异。结论 用含H2S的St.Thomas Ⅱ液低温保存大鼠离体心脏，8 h和10 h后心肌组织均出现中度损伤，保存10 h再灌注后出现严重的心功能障碍，而保存8 h以内的离体心脏的保存效果良好。
Abstract:
AIM To investigate the ultrastructural changes of isolated rat hearts after hypothermic preservation by St.Thomas Ⅱ solutions containing H2S for different periods of time and reperfusion for 30 minutes. METHODS Thirtytwo isolated rat hearts were randomly divided into four groups (n=8 each) and the hearts were arrested with St. Thomas Ⅱ plus hydrogen sulfide solution and preserved at 4℃ hypothermically in the same solution for 4 h, 6 h, 8 h and 10 h. The isolated hearts were perfused on the Langendorff perfusion apparatus for functional evaluation before and after preservation. Myocardial enzyme leakage and cardiac ultrastructure were measured after reperfusion. RESULTS The recovery of myocardial function decreased with the extension of preservation time and myocardial enzyme leakage increased gradually. Cardiac ultrastructure presented a minor injury after the hearts were preserved for 4 h and 6h and presented secondary injury after 8 h and 10 h. After reperfusion, the difference of the recovery of myocardial function was significant between 8 h group and 10h group. CONCLUSION When isolated hearts are preserved in St.Thomas Ⅱ solutions containing H2S, the hearts could be well preserved within 8 h preservation. Secondary injury of myocardium tissue would appear after 8 h and 10h preservation and the function of the heart would be seriously impaired after 10h preservation.

参考文献/References

［1］耿彬，杜军保，唐朝枢. 内源性H2S—一种新的气体信号分析［J］. 生理科学进展，2002，33(3)：255-258.

［2］Zhao W, Zhang J, Lu Y, et al. The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener ［J］. EMBO J, 2001, 20(21): 6008-6016.

［3］Blackstone E，Morrison M，Roth MB. H2S Induces Suspended AnimationLike State in Mice ［J］. Science，2005,308(5721）:518.

［4］Hachida M, Lu H, Ohkado A, et al. Effect of ATPpotassium channel opener nicerandial on longterm cardiac preservation［J］. J Cardiovasc Surg (Torino), 2000, 41(4): 533-539.

［5］Ych CH , Wang YC , Wu YC , et al. Continuous tepid blood cardioplegia can preserve coronary endot helium and ameliorate the occurrence of cardiomyocyte apoptosis［J］ . Chest , 2003 ,123 (5) : 1647-1654.

［6］Parolari A , Rubini P , Cannata A , et al. Endothelial damage during myocardial preservation and storage［J］ . Ann Thorac Surg , 2002 , 73 (2) : 682-690.

备注/Memo

备注/Memo:
收稿日期：2007-03-05.作者简介：白崇峰，主治医师，硕士Email:baichf@.fmmu.edu.cn
更新日期/Last Update: