我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

前胡丙素对缺血/再灌注过程中心肌细胞膜钙调节蛋白的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第5期
页码:
513-516
栏目:
基础研究
出版日期:
2008-10-20

文章信息/Info

Title:
Effects of praeruptorin C on myocardial plasma membrane calcium handling proteins during ischemia/reperfusion
作者:
奚群英; 祝宝华; 刘晨; 张能锋
东南大学医学院附属扬州医院心内科，江苏 扬州 225001
Author(s):
XI Qunying;  ZHU Baohua;  LIU Chen;  ZHANG Nengfeng
Department of Cardiology, Yangzhou Hospital Affiliated to Medical School, Southeast University, Yangzhou 225001, Jiangsu, China
关键词:
前胡丙素; 缺血/再灌注损伤; 心肌; 钠钙交换蛋白
Keywords:
praeruptorin C;  ischemia/reperfusion injury;  myocardium;  natriumcalcium exchanger
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的 探讨中药单体前胡丙素（Pra-C）对缺血/再灌注(I/R)心肌细胞保护作用的机制。方法 将培养的乳鼠心肌细胞随机分为I/R组、Pra-C预处理组和对照组。用全自动生化仪测定各组乳酸脱氢酶（LDH）的漏出量（n＝5）。半定量RTPCR检测钠钙交换蛋白（NCX）mRNA的转录（n＝3）,其活性以液闪仪测定Na+依赖的45Ca2+摄取率表示(n＝5)。细胞膜钙ATP酶(PMCA)的活性以定磷比色法测定(n＝5)。结果 I/R组LDH的漏出量显著增加(P<0.01),PraC预处理组与I/R组比较LDH的漏出量显著降低（P<0.01）。I/R组NCX Na+依赖的45Ca2+摄取率比对照组显著增高（P<001），Pra-C预处理组较I/R时45Ca2+摄取率降低（P<001）。I/R组NCX mRNA的水平比对照组显著高（P<0.01）；Pra-C预处理组较I/R组NCX mRNA的水平显著低（P<0.01）。各组间PMCA的活性无显著性差异。结论 Pra-C可显著减轻I/R损伤；其对I/R心肌的保护与下调细胞膜NCX mRNA的转录和其蛋白的活性有关。
Abstract:
AIM To explore the cardioprotective mechanism of praeruptorin C （PraC）during ischemia/reperfusion. METHODS Cultured rat neonatal cardiomyocytes were randomly divided into 3 groups: ischemia/reperfusion group (9 h ischemia followed by 1 h reperfusion, I/R ), PraC pretreatment group (pretreated with PraC for 1 h before I/R) and control group. The leakage of intracellular lactate dehydrogenase (LDH) in various groups was determined by biochemical autoanalyzer. Activity of natriumcalcium exchanger(NCX) was indicated by Na+dependent 45Ca2+ uptake tested by liquid scintillation counting. Semiquantitative RTPCR was employed to detect the mRNA level of NCX. Activity of plasma membrane calcium ATPase (PMCA) was determined by microcolorimetrg of inorganic phosphorus. RESULTS Compared with that in I/R group, the LDH leakage in PraC pretreatment group was significantly reduced (P<001). The Na+dependent 45Ca2+ uptake was significantly increased in I/R group (P<001) compared with that in control group. This increase could be significantly attenuated in PraC pretreatment group. The level of NCX mRNA in I/R group was also significantly increased (P<001) compared with that in control group and this increase could be significantly attenuated in PraC pretreatment group (P<001). No significant change of PMCA activity was observed between various groups. CONCLUSIONN CX mediates the cardioprotective effect of PraC during ischemia/reperfusion in the neonatal rat cardiomyocytes I/R model.

参考文献/References

［1］ Chen L, Lu XY, Li J, et al. Intermittent hypoxia protects cardiomyocytes against ischemiareperfusion injuryinduced alterations in Ca2+ homeostasis and contraction via the sarcoplasmic reticulum and Na+/Ca2+ exchange mechanisms ［J］. Am J Physiol Cell Physiol, 2006, 290(4):C1221-C1229.

［2］ Yang JR, Li QP, Rao MR. Effects of praeruptorin C on hemodynamics of isolated rat heart with ischemiareperfused damage ［J］. Chin J Pharmacol Toxicol, 1995, 9(4):271-273.

［3］ Xia Y, Rajapurohitam V, Cook MA, et al. Inhibition of phenylephrine induced hypertrophy in rat neonatal cardiomyocytes by the mitochondrial KATP channel opener diazoxide ［J］. J Mol Cell Cardiol, 2004, 37(5):1063-1067.

［4］ Nawada R, Murakami T, Iwase T, et al. Inhibition of sarcolemmal Na+, K+ATPase activity reduces the infarct sizelimiting effect of preconditioning in rabbit hearts［J］. Circulation, 1997, 96(2):599-604.

［5］ Trafford AW, Diaz ME, Eisner DA. Coordinated control of cell Ca2+ loading and triggered release from the sarcoplasmic reticulum underlies the rapid inotropic response to increased Ltype Ca2+ current［J］. Circ Res, 2001, 88(2):195-201.

［6］ Wu X, Rao MR. Effects of praeruptorin C on isolated guinea pig atrium and myocaridal compliance in patients［J］. Zhongguo Yao Li Xue Bao, 1990， 11（3）：235-238.

［7］ Yang JR, Li QP, Rao MR. Protective effects of praeruptorin C and nifedipine on ischemiareperfused injury in working rat hearts［J］. Yao Xue Xue Bao, 1992, 27（10）：729-733.

［8］ Wang HX, Rao MR, Wang JX, et al. Effect of praeruptorin C on spontaneous ［Ca2+］i transients in cultured myocardial cells of neonatal rats ［J］. Acta Pharmacol Sinica, 1997, 18(1): 81-84.

［9］ Sun L, Rao MR, Wang JX. Effects of praeruptorine C on the intracellular free calcium in normal and hypertrophied rat ventricular myocytes ［J］. Zhongguo Yao Li Xue Bao, 1997, 18(3):251-254.

［10］Li SZ, Wu F, Wang B, et al. Role of reverse mode Na+/Ca2+ exchanger in the cardioprotection of metabolic inhibition preconditioning in rat ventricular myocytes ［J］. Eru J Pharmacol, 2007, 561(1-3):14-22.

［11］Wang J, Zhang Z, Hu Y, et al. SEA0400, a novel Na+/Ca2+ exchanger inhibitor, reduces calcium overload induced by ischemia and reperfusion in mouse ventricular myocytes［J］. Physiol Res, 2007, 56(1):17-23.

［12］Reuter H, Henderson SA, Han T, et al. Cardiac excitationcontraction coupling in the absence of Na+Ca2+ exchange［J］. Cell Calcium, 2003, 34(1):19-26.

［13］Henderson SA, Goldhaber JI, So JM, et al. Functional adult myocardium in the absence of Na+Ca2+ exchange: cardiacspecific knockout of NCX1［J］. Circ Res, 2004, 95(6):604-611.

备注/Memo

备注/Memo:
收稿日期：2007-05-09.基金项目：江苏省135工程医学重点人才基金项目资助（RC2002016） 通讯作者：祝宝华，教授，主要从事冠心病介入治疗研究Email:yzbaohua@yahoo.com 作者简介：奚群英，住院医师，硕士Email:xiqunying@yahoo.com.cn
更新日期/Last Update: