我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

缺血预适应对SD大鼠缺血/再灌注血清TNF-α、IL-8和心肌IL-10水平的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2008年第5期
页码:
517-520
栏目:
基础研究
出版日期:
2008-10-20

文章信息/Info

Title:
Effect of ischemic preconditioning on tumor necrosis factorα interleukin8 and interleukin10 in SpraqueDawley rats following myocardial ischemia and reperfusion
作者:
方忠林; 谢志泉
广州军区总医院干部病房四科,广东 广州 510010
Author(s):
FANG Zhonglin;  XIE Zhiquan
Fourth Department of Geriatrics, PLA Guangzhou General Hospital, Guangzhou Military Area Command, Guangzhou 510010, Guangdong, China
关键词:
缺血预适应; 肿瘤坏死因子-α; 白细胞介素-8; 白细胞介素-10; 心肌缺血/再灌注
Keywords:
ischemic preconditioning;  tumor necrosis factorα;  interleukin8;  interleukin10;  myocardial ischemiareperfusion
分类号:
-
DOI:
-
文献标识码:
A
摘要:
目的 观察缺血预适应对SD大鼠心肌缺血/再灌注期血清中肿瘤坏死因子-α（TNF-α）和白细胞介素-8(IL-8)及心肌组织中的白细胞介素-10(IL-10)的影响，并探讨其心肌保护机制。方法 36只SD大鼠随机分成3组：①缺血预适应（IPC）组：缺血5 min+再灌注5 min反复4次后，缺血60 min+再灌注120 min；②缺血/再灌注（IRI）组：大鼠开胸后，继续观察40 min，然后予以缺血60 min+再灌注120 min；③假手术组（SO）：仅开胸，分离血管，不予缺血与再灌注剌激，只进行同样长时间的观察。3组实验结束取右心房血及心肌组织，批量测定血清TNF-α和IL-8及心肌组织IL-10浓度。结果 IRI组血清TNF-α和IL-8含量分别为（1 009±277）和（334±187）ng/L，IPC组血清TNFα和IL-8含量分别为（778±178）和（229±90）ng/L，较IRI组显著降低（P<0.05）；IRI组心肌组织IL-10含量为（6.8±2.6）ng/g，IPC组心肌组织IL10含量为（9.4±4.1）ng/g，较IRI组显著升高（P<0.05）。结论 缺血预适应不仅可减少心肌缺血/再灌注病理过程中促炎细胞因子TNF-α和IL-8的产生,还可促进抗炎细胞因子IL-10的产生,而发挥抗炎作用,提示缺血预适应具有保护再灌注心肌抗炎作用的新机制。
Abstract:
AIM To observe the effects of ischemic preconditioning on the production of tumor necrosis factorα (TNFα), interleukin8 (IL8) and interleukin10 (IL10) following myocardial ischemia and reperfusion and to investigate its mechanism. METHODS Thirtysix SpraqueDawley rats were randomly divided into three groups: ischemic preconditioning group (group IPC, n=12) was subjected to a 5 min occlusion of left anterior descending coronary artery (LAD), followed by reperfusion for 5 min four times and then elicited by 1h occlusion and 2 h reperfusion; ischemia reperfusion group (group IRI, n=12) was observed for 40 min and then elicited by 1 h occlusion and 2 h reperfusion; shamoperated group (group SO, n=12) was only observed for 40 min. At the end of the experiment, the right atrium myocardial blood and myocardial tissue were obtained and the serum TNFα, IL8 mass and myocardial tissue concentrations of IL10 were detected. RESULTS In group IRI, the serum levels of TNFα and IL8 were (1 009±277) and (334±187)ng/L and inn group IPC, the serum levels of TNFα and IL8 were (778±178) and (229± 90)ng/L, significantly lower than those in IRI group (P<005). In group IRI, the myocardial IL10 was (68±26) ng/g and in group IPC, the myocardial IL10 was (94±41)ng/g, significantly higher than those in IRI group (P<005)CONCLUSION Ischemic preconditioning not only reduces the myocardial ischemiareperfusion pathological process of proinflammatory cytokines TNFα and the IL8 produce, but also promotes the antiinflammatory cytokines IL10, suggesting that the protective effect of ischemic preconditioning on myocardial reperfusion injury through antiinflammatory mechanism.

参考文献/References

［1］ Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium［J］. Circulation, 1986, 74(5):1124-1136.

［2］ Squadrito F, Altavilla D, Zingarelli B, et al. Tumor necrosis factor involvement in myocardial ischemiareperfusion injury［J］. Eur J Pharmacol, 1993, 237(2-3):223-230.

［3］ Kukieika GL, Smith CW, Larosa GJ, et al. IL8 gene in the myocardium after ischemia and reperfusion in vivo［J］. Clin Invest, 1995, 95(1):89.

［4］ 吴付轩，丁文惠，张钧华，等. 急性心肌缺血再灌注期间血浆白介素8的变化和意义［J］. 中国实用内科杂志, 1999， 19（9）:527-529.

［5］ 盛西陵，王东明，陈畅，等. 内源性白介素10在心肌缺血-再灌注损伤中的作用 ［J］. 中华急诊医学杂志， 2004 ， 13（10）:676-678.

［6］ Yang Z, Zingarelli B, Szabo C. Crucial role of endogenous interleukin10 production in myocardial ischemia/reperfusion injury［J］. Circulation, 2000, 101(9):1019-1026.

［7］ Samoilova EB, Horton JL, Hilliard B, et al. IL6 deficient mice are resistant to experimental autoimmune encephalomyelitis: roles of IL6 in the activation and differentiation of autoreactive T cells［J］. Immunol, 1998, 161(12):6480-6486.

［8］ Pahan K, Sheikh FG, Namboodiri AM, et al. Lovastatin and phenylacetate inhibit the induction of nitric oxide synthase and cytokines in rat primary astrocytes, microglia and macrophages［J］. J Clin Invest, 1997, 100(11):2671-2679.

［9］ Beg AA, Sha WC, Bronson RT, et al. Embryonic lethality and liver degeneration in mice lacking the RelA component of NFkappa B［J］. Nature, 1995, 376(6536):167-170.

［10］吴刚，杨蕾，刘永锋，等. 缺血预处理对大鼠肝脏缺血再灌注损伤保护作用机制的研究［J］. 中华肝胆外科杂志， 2003， 9（4）:221-224.

备注/Memo

备注/Memo:
收稿日期：2006-10-12.基金项目:广东省自然科学基金项目资助（20010783） 通讯作者：谢志泉，副教授，博士，主要从事缺血预适应对心肌微循环研究Email：xie1zq@163.com 作者简介：方忠林，主治医师，硕士生Email：w7851381@yahoo.com.cn
更新日期/Last Update: