我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

葡萄糖浓度波动对人外周血内皮祖细胞氧化应激的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第1期
页码:
18
栏目:
基础研究
出版日期:
2009-02-28

文章信息/Info

Title:
Effects of glucose concentration fluctuations on oxidative stress in peripheral blood endothelial progenitor cells
作者:
吴跃春1; 任雨笙1; 梁春1; 谭鸿斌1; 张鹏2; 吴宗贵1
第二军医大学第二附属医院：1.心内科,2.神经内科，上海 200003
Author(s):
WU Yue-chun1;  REN Yu-sheng1;  LIANG Chun1;  TAN Hong-bin1;  ZHANG Peng2;  WU Zong-gui1
1.Department of Cardiology, 2.Department of Neurology, Second Affiliated Hospital, Second Military Medical University, Shanghai 200003, China
关键词:
葡萄糖; 人内皮祖细胞; 氧化应激; 活性氧
Keywords:
glucose;  human endothelial progenitor cells;  oxidative stress;  reactive oxygen species
分类号:
R329.2
DOI:
-
文献标识码:
A
摘要:
目的 观察葡萄糖浓度波动对人外周血内皮祖细胞（EPCs）活性氧水平的影响。方法 采用密度梯度离心法分离外周血单个核细胞，培养7 d后，收集贴壁细胞，进行FITC-UEA-Ⅰ、DiI-acLDL双染色鉴定，并用流式细胞术检测细胞的表面标志CD34、CD133和血管内皮生长因子受体-2。EPCs给予不同浓度波动幅度(0、10、20 mmol/L)、不同浓度波动间期(3、6、12 h)、不同平均浓度(20、25、30 mmol/L)的葡萄糖，培养一定时间(6、12、24、48、96 h)后，以氯甲基二氯二氢荧光素二乙酯染色细胞，用流式细胞术检测细胞内平均荧光强度(MFI)。结果 葡萄糖浓度波动幅度越大、浓度波动间期越小、平均浓度越高，细胞内MFI越高(P<0.05)。平均浓度对MFI的影响随时间减小；而浓度波动对MFI的影响随时间增大（P<0.05）。结论 葡萄糖浓度波动在葡萄糖所致人外周血EPCs慢性氧化应激中起重要作用。
Abstract:
AIM To observe the effect of glucose concentration fluctuations on the active oxygen level in peripheral blood endothelial progenitor cells (EPCs). METHODS Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation. After the cells were cultured for 7 days, the cells adhering to culture dish were characterized by FITC-UEA-I and DiI-acLDL double staining under a laser scanning confocal microscope. EPCs were further documented by demonstrating the expression of CD34, CD133 and VEGFR-2 with flow cytometry. Several groups of attached cells were incubated with glucose in a series of fluctuant amplitudes (0, 10 and 20 mmol/L), fluctuant intervals (3, 6 and 12 h) and mean concentrations (20, 25 and 30 mmol/L) for different durations (6, 12, 24, 48 and 96 h). A probe, chloromethyl derivative of dichlorodihydrofluorescein diacetate (CM-H2DCFDA) used to evaluate intracellular reactive oxidative species production, was incubated with EPCs for 20 minutes. The mean fluorescence intensity was determined in alive EPCs with flow cytometry. RESULTS Intracellular mean fluorescence intensity was positively proportional to fluctuant amplitude of glucose concentration and average concentration of glucose (P<0.05). The smaller the fluctuant interval of glucose concentration was, the higher was the mean fluorescence intensity (P<0.05). With the passage of time, the influence of average concentration of glucose on the intracellular mean fluorescence intensity gradually decreased and the influence of glucose fluctuations gradually increased (P<0.05). CONCLUSION Concentration of glucose fluctuations plays an important role in the chronic oxidative stress produced by glucose in EPCs of human peripheral blood.

参考文献/References

[1］ Quagliaro L, Piconi L, Assaloni R, et al. Intermittent high glucose enhances apoptosis related to oxidative stress in human umbilical vein endothelial cells: the role of protein kinase C and NAD(P)Hoxidase activation［J］. Diabetes, 2003, 52(11):2795-2804.
［2］ Brownlee M. The pathobiology of diabetic complications:a unifying mechanism［J］. Diabetes, 2005, 54(6):1615-1625.
［3］ 周健,喻明,贾伟平,等. 应用动态血糖监测系统评估2型糖尿病患者日内及日间血糖波动幅度［J］. 中华内分泌代谢杂志, 2006, 22(3):286-288.
［4］ Glucose tolerance and mortality: comparison of WHO and American Diabetes Association diagnostic criteria. The DECODE study group. European Diabetes Epidemiology Group. Diabetes Epidemiology: Collaborative analysis Of Diagnostic criteria in Europe［J］. Lancet, 1999, 354(9179):617-621.
［5］ Hirsch IB. Glycemic variability： it’s not just about HbA1c anymore［J］. Diabetes Technol Ther, 2005, 7(5):780-783.
［6］ Risso A, Mercuri F, Quagliaro L, et al. Intermittent high glucose enhances apoptosis in human umbilical vein endothelial cells in culture［J］. Am J Physiol Endocrinol Metab, 2001, 281(5):924-930.
［7］ Tepper OM, Galiano RD, Capla JM, et al. Human endothelial progenitor cells from type II diabetes exhibit impaired proliferation, adhesion, and incorporation into vascular structures［J］. Circulation, 2002, 106(22):2781-2786.
［8］ Krankel N, Adams V, Linke A, et al. Hyperglycemia reduces survival and impairs function of circulating bloodderived progenitor cells［J］. Arterioscler Thromb Vasc Biol, 2005, 25(4):698-703.
［9］ Callaghan MJ, Ceradini DJ, Gurtner GC. Hyperglycemiainduced reactive oxygen species and impaired endothelial progenitor cell function［J］. Antioxid Redox Signal, 2005, 7(11-12):1476-1482.
［10］Chen YH, Lin SJ, Lin FY, et al. High glucose impairs early and late endothelial progenitor cells by modifying nitric oxiderelated but not oxidative stressmediated mechanisms［J］. Diabetes, 2007, 56(6):1559-1568.
［11］Piconi L, Quagliaro L, Assaloni R, et al. Constant and intermittent high glucose enhances endothelial cell apoptosis through mitochondrial superoxide overproduction［J］. Diabetes Metab Res Rev, 2006, 22(3):198-203.
［12］Monnier L, Mas E, Ginet C, et al. Activation of oxidative stress by acute glucose fluctuations compared with sustained chronic hyperglycemia in patients with type 2 diabetes［J］. JAMA, 2006, 295(14):1681-1687.

备注/Memo

备注/Memo:
收稿日期：2008-3-24.基金项目：国家973课题基金项目资助(2005CB523309) 通讯作者：任雨笙，副主任医师，主要从事冠心病临床及基础研究Email:renyusheng@gmail.com 作者简介：吴跃春，主治医师，硕士生Email:wyc7103@yahoo.com.cn
更新日期/Last Update: 2009-04-02