绿茶多酚影响高脂饮食大鼠窖蛋白-1表达的实验研究
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2010年第1期
- 页码:
- 37-39
- 栏目:
- 基础研究
- 出版日期:
- 2010-01-04
文章信息/Info
- Title:
- Experimental study on effect of epigallcatechin gallate on caveolin-1 expression in rats fed a high-fat diet
- Author(s):
- XIONG Ri-cheng; YU Zhou; LAI Tian-shun
- Medical Intensive Care Unit, Guangzhou General Hospital, Guangzhou Military Area Command, Guangzhou 510010, Guangdong, China
- 关键词:
- 高脂饮食; 肥胖; 窖蛋白-1; 表没食子儿茶素没食子酸酯; 大鼠
- Keywords:
- high-fat diet; obesity; caveolin-1; epigallcatechin gallate; rats
- 分类号:
- R51.1
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 探讨绿茶多酚单体表没食子儿茶素没食子酸酯(epigallcatechin gallate，EGCG)对高脂饮食所致肥胖及窖蛋白-1（caveolin-1，Cav-1）表达的影响。方法: 30只雄性Wistar大鼠（体质量170~190 g）随机分为3组，即高脂饮食组、高脂饮食加EGCG组和对照组。饲养15周，测量体质量、血糖及血脂；用RT-PCR及Western blot分别检测Cav-1 mRNA及其蛋白表达。结果: 高脂饮食可上调Cav-1 mRNA和其蛋白表达；而EGCG可降低Cav-1 mRNA和其蛋白的上调。结论: 绿茶多酚降低高脂饮食大鼠Cav-1 mRNA和其蛋白表达的作用,可能是绿茶及其多酚改善内皮功能和预防心血管疾病的分子机制之一。
- Abstract:
- AIM: To investigate the effects of epigallcatechin gallate (EGCG) on high-fat diet-related obesity and on vascular caveolin-1 (Cav-1) expression. METHODS: Male Wistar rats weighing 170-190 g were randomly divided into three groups: one group fed with a high-fat diet for 15 weeks, the second group fed with a high-fat diet plus EGCG ［5 mg/(kg·day)］ by abdominal cavity injection and the control group fed with a general diet for 15 weeks. Weight, serum glucose and lipid concentrations were measured and Cav-1 expression was determined using reverse transcription (RT)-PCR and Western blotting. RESULTS: A high-fat diet up-regulated Cav-1 and these effects were partially reversed in EGCG group. CONCLUSION: These findings suggest that EGCG down-regulates Cav-1 expression in rats fed a high-fat diet, which may result from the molecular mechanism of tea in amelioration of endothelial function and prevention of angiocardiopathy.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2009-1-4.通讯作者：俞宙，副主任医师，主要从事冠心病的基础及介入治疗研究Email：yuzhoumicu@163.com 作者简介：熊日成,硕士生Email:xricheng@163.com
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