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|本期目录/Table of Contents|

替米沙坦对代谢综合征大鼠血管反应性及解偶联蛋白-2表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第1期
页码:
40-43,47
栏目:
基础研究
出版日期:
2010-01-04

文章信息/Info

Title:
Effect of telmisartan on vascular reactivity and expression of uncoupling protein 2 in rats with metabolic syndrome
作者:
张鑫; 杨大春; 杨永健; 李德; 唐兵
成都军区总医院心血管内科，四川 成都 610083
Author(s):
ZHANG Xin;  YANG Da-chun;  YANG Yong-jian;  Li De;  TANG Bing
Department of Cardiology, Chengdu General Hospital, Chengdu Military Area Command, Chengdu 610083, Sichuan, China
关键词:
替米沙坦; 代谢综合征; 血管功能; 解偶联蛋白-2; 大鼠
Keywords:
telmisartan;  metabolic syndrome;  vascular reactivity;  uncoupling protein;  rats
分类号:
Q514.3
DOI:
-
文献标识码:
A
摘要:
目的: 探讨血管紧张素Ⅱ受体拮抗剂替米沙坦(Telm)对代谢综合征（MS）大鼠血管功能和解偶联蛋白-2(UCP-2)表达的影响。方法: 将45只2月龄的雄性Wistar大鼠，随机分为普食对照组、MS组和MS+Telm组，每组15只，喂养6月，测定鼠尾的血压。硝酸还原酶法检测血浆一氧化氮(NO)浓度，羟胺法测定超氧化物歧化酶(SOD)浓度，硫代硫酸巴比妥法(TBA法)测定丙二醛(MDA)水平，检测采用相应的试剂盒。取大鼠胸主动脉，利用体外血管环张力测定技术，检测其对苯肾上腺素(PE)、乙酰胆碱(Ach)和硝普钠(SNP)的反应。应用Western blot法检测胸主动脉UCP-2和内皮型一氧化氮合酶(eNOS)的表达。结果: MS+Telm组体外胸主动脉对PE诱导的收缩反应明显低于MS组(P<0.05)，对Ach诱导的舒张反应明显高于MS组(P<0.05)。MS+Telm组血浆SOD、NO的水平高于MS组，MDA的水平低于MS组(P<0.05)，其胸主动脉UCP-2、eNOS的表达明显高于MS组(P<0.05)。结论: Telm能促进主动脉中UCP-2和eNOS的表达，有效改善MS大鼠血管的反应性。
Abstract:
AIM: To investigate the effects of angiotensin II receptor antagonist telmisartan on the vascular reactivity and expression of uncoupling protein 2 in rats with metabolic syndrome (MS). METHODS: Male wistar rats, aged 2-month-old, were divided into control group, MS group and MS+telmisartan (MS+Telm) group. Telmisartan was dissolved in 1 ml drinking water at a dosage of 5 mg/kg per day by stomach intubations using a round-ended needle for 6 months. Tail-cuff systolic blood pressure (SBP) was measured. At the end of the treatment period, plasma nitric oxide (NO), superoxide dismutase (SOD) and malondialdehyde (MDA) were detected. Aortic relaxation and contraction were examined using organ bath connecting with polygraphy. eNOS protein and uncoupling protein 2 (UCP-2) expression were measured by Western blot. RESULTS: PE-induced contractile response of thoracic aorta in MS+Telm group was significantly lower than in MS group (P<0.05), whereas acetylcholine (Ach)-induced relaxation response was higher than in MS group (P<0.05). Levels of plasma SOD and NO in MS+Telm group were higher than in the MS group but levels of plasma MDA were lower than in the MS group. Administration of telmisartan significantly increased UCP-2 and eNOS protein expression in aortic rings (P<0.05). CONCLUSION: Telmisartan promotes UCP-2 and eNOS expression in thoracic aorta and effectively improves vascular function in rats with MS.

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备注/Memo

备注/Memo:
收稿日期：2009-2-12.通讯作者：张鑫，主任医师，主要从事冠心病基础与临床研究Email:zhangx_ok@126.com
更新日期/Last Update: 2010-01-05