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|本期目录/Table of Contents|

Ghrelin对大鼠心肌微血管内皮细胞的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第4期
页码:
506-509,516
栏目:
基础研究
出版日期:
2010-06-10

文章信息/Info

Title:
Protective effects of ghrelin on rat coronary microvascular endothelial cells
作者:
王亚斌; 孙冬冬; 赵力; 张铮; 张荣庆; 曹丰; 王海昌
第四军医大学西京医院心脏内科，陕西 西安 710032
Author(s):
WANG Ya-bin;  SUN Dong-dong;  ZHAO Li;  ZHANG Zheng;  ZHANG Rong-qing;  CAO Feng;  WANG Hai-Chang
Department of Cardiovascular Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
ghrelin; 心肌微血管内皮细胞; 细胞增殖; 细胞迁移; 一氧化氮
Keywords:
ghrelin;  cardiac microvascular endothelial cell;  cell proliferation;  nitric oxide
分类号:
R972
DOI:
-
文献标识码:
A
摘要:
目的: 探讨ghrelin对大鼠心肌微血管内皮细胞(cardiac microvascular endothelial cells，CMECs)增殖、迁移能力和分泌NO的影响。方法: 以酶消化法获得SD大鼠的CMECs，采用Dil-ac-LDL吞噬试验鉴定CMECs。将新一代CMECs分5组分别干预24 h：即(A)空白对照组、(B)1×10-9 mol/L ghrelin组、(C)1×10-8 mol/L ghrelin组、(D)1×10-7 mol/L ghrelin组和(E)ghrelin+拮抗剂LY294002组（1×10-7 mol/L ghrelin和20 μmol/L LY294002）。用MTT比色法检测ghrelin对CMECs增殖的影响；以transwell法检测ghrelin对CMECs迁移的影响。用硝酸还原法检测CMECs分泌NO的量。结果: Dil-ac-LDL吞噬试验表明，90%以上的CMECs表达Dil-ac-LDL。与对照组相比，10-8~10-7 mol/L的ghrelin可明显促进CMECs增殖和迁移，以及NO分泌(P<0.05)；LY294002则能够明显抑制ghrelin对CMECs的促增殖和迁移作用，以及抑制ghrelin刺激CMECs分泌NO的作用（P>0.05）。结论: 一定浓度条件下，ghrelin能够促进心肌微血管内皮细胞（CMEC）的增殖、迁移和NO分泌，这可能与激活AKT/PI3K信号转导通路有关。
Abstract:
AIM: To investigate the effects of ghrelin on the proliferation, migration and nitric oxide (NO) secretion in rat cardiac microvascular endothelial cells (CMECs). METHODS: CMECs were isolated from rat left ventricles by enzyme digestion and cultured in fibronectin-coated dishes. The obtained CMECs were identified by Dil-ac-LDL phagocytosis and collected cells were divided into control group, groups with different doses of ghrelin (1×10-9 mol/L, 1×10-8 mol/L, 1×10-7 mol/L) and ghrelin + antagon LY294002 group (1×10-7 mol/L ghrelin+20 mol/L LY294002). After 24 h exposure, proliferation capability was measured by MTT assay, migration of CMECs was detected by transwell assay and NO secretion of CMECs was measured by nitrate reduction. RESULTS: CMECs were successfully obtained by enzyme digestion and ghrelin at 10-8-10-7 mol/L promoted proliferation, migration and NO secretion of CMECs (P<0.05). However, LY294002 inhibited the ghrelin-promoting proliferation and migration of CMECs and decreased ghrelin-stimulating NO secretion of CMECs. CONCLUSION: Ghrelin can promote proliferation, migration and NO secretion of rat coronary microvascular endothelial cells at some dose. This may be related to the activation of AKT/PI3K signal pathway.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-07-22.通讯作者：王海昌，主任医师，主要从事冠心病的机制和治疗研究Email:wanghc@fmmu.edu.cn 作者简介：王亚斌，住院医师，硕士生Email:scottss0415@gmail.com
更新日期/Last Update: 2010-05-20