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|本期目录/Table of Contents|

肝素涂层体外循环管道中聚乙烯亚胺的作用研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第5期
页码:
670-672,676
栏目:
基础研究
出版日期:
2010-06-22

文章信息/Info

Title:
Effects of concentration and molecular weight of polyethyleneimine on heparin-coated modified polyvinyl chloride material
作者:
杨春1; 杨剑1; 易定华1; 罗鹏1; 曹瑞军2; 周华2
1.第四军医大学西京医院心血管外科，陕西 西安 710032;2.西安交通大学应用化学系，陕西 西安 710049
Author(s):
YANG Chun1;  YANG Jian1;  YI Ding-hua1;  Luo Peng1;  Cao Rui-jun2;  Zhou Hua2
1.Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China; 2.Deptartment of Applied Chemistry, Xi’an Jiaotong University, Xi’an 710049, Shaanxi, China
关键词:
聚乙烯亚胺; 体外循环管道; 肝素涂层
Keywords:
polyethyleneimine;  polyvinyl chloride-heparin coating
分类号:
R978.17
DOI:
-
文献标识码:
A
摘要:
目的: 探讨聚乙烯亚胺(PEI)的浓度、平均相对分子量对涂层肝素的影响，筛选出合适的各种底物的浓度及反应条件，优化涂层工艺。方法: 将PEI的平均相对分子量（3 000、10 000、20 000）和质量浓度（1 g/dl、2 g/dl、5 g/dl）交叉组合分为9个组，制成PEI-戊二醛-肝素涂层体外循环管道。利用红外光谱对结合肝素的表征定性，用甲苯胺蓝分光光度法测量肝素的结合含量。结果: 通过PEI-戊二醛-肝素涂层体外循环管道肝素的结合量，受PEI的分子量及浓度的影响，最适质量浓度及平均相对分子量分别为2 g/dl和20 000。结论: 各组肝素涂层材料肝素的浓度随PEI平均相对分子量的递增而增加，在一定范围内，PEI的浓度与肝素的含量呈正相关，浓度过高或过低均不利于肝素的结合。
Abstract:
AIM：To study the effects of concentration and molecular weight of polyethyleneimine (PEI) on PEI-heparin-coated polyvinyl chloride (PVC) medical materials. METHODS: Using PEI-glutaraldehyde-heparin coating techniques, cardiopulmonary bypass (CPB) circuits were coated with heparin and then divided into nine groups by PEI concentrations and molecular weights. The content of the coated heparin was calculated by toluidine blue spectrophotometry. RESULTS: The combination of the amount of heparin on PEI-heparin-coated PVC medical material was affected by the concentration and molecular weight of PEI. The optimal concentration and molecular weight of PEI were 2 g/dl and 20 000, respectively. CONCLUSION: Heparin concentration on PEI-glutaraldehyde-heparin-coated PVC surface is increased by the concentration and molecular weight of PEI. The increase is to some degree positively related to the content of PEI. Too high or too low PEI concentrations are not conducive to the combination of heparin.

参考文献/References

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［2］Wendel HP, Ziemer G. Coating-techniques to improve the hemocompatibility of artificial devices used for extracorporeal circulation［J］. Eur J Cardiothorac Surg, 1999, 16(3): 342-350.

［3］杨剑,易定华,刘金成,等. 肝素涂层体外循环管道的制备及生物学性能评价［J］.中国生物医学工程学报, 2004, 23（2）:169-174.

［4］Hsu LC. Biocompatibility in cardiopulmonary bypass［J］. J Cardiothoracic Vasc Anesth, 1997, 11(3):376-382.

［5］张安兄,吕德龙,程为庄,等. 生物材料的血液相容性［J］.上海生物医学工程, 2004, 25(3):53-58.

［6］杨剑,易定华. 体外循环中的肝素涂层技术［J］. 国外医学·生物医学工程分册, 2003, 26(2):89-93.

［7］Huang LY, Yang MC. Hemocompatibility of layer-by-layer hyaluronic acid/heparin nanostructure coating on stainless steel for cardiovascular stents and its use for drug delivery［J］. J Nanosci Nanotechnol, 2006, 6(9-10):3163-3170.

［8］Thorslund S, Sanchez J, Larsson R, et al. Functionality and stability of heparin immobilized onto poly(dimethylsiloxane)［J］. Colloids Surf B Biointerfaces, 2005, 45(2):76-81.

［9］Lappegard KT, Riesenfeld J, Brekke OL, et al. Differential effect of heparin coating and complement inhibition on artificial surface-induced eicosanoid production［J］. Ann Thorac Surg, 2005, 79(3):917-923.

［10］杨剑,易定华,钟丽辉,等. 肝素涂层体外循环管道抗凝血性能的研究［J］. 生物医学工程与临床, 2003, 7(2):100-103.

[11]杨剑,易定华,刘金成,等. MTT法评价肝素涂层医用聚氯乙烯的细胞毒性 ［J］. 生物医学工程与临床, 2004, 8(1):11-13.

[12]杨剑,易定华,刘金成,等. 肝素涂层医用聚氯乙烯的细胞毒性研究 ［J］. 生物医学工程学杂志, 2005, 22(4):739-741.

备注/Memo

备注/Memo:
收稿日期：2009-08-01.基金项目：国家自然科学基金项目资助(30700174)；陕西省社发攻关基金课题(2006K12-G5(4)］项目资助 通讯作者：易定华，教授，主要从事心血管外科学研究Email:yidh@fmmu.edu.cn 作者简介：杨春，硕士生Email:yangchunchenjuan@yahoo.cn
更新日期/Last Update: 2010-06-22