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|本期目录/Table of Contents|

Ghrelin注射对大鼠血压和心肌收缩功能的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第5期
页码:
673-676
栏目:
基础研究
出版日期:
2010-06-22

文章信息/Info

Title:
Effects of intravenous injection of ghrelin on blood pressure and cardiac contractility in rats
作者:
曹钰琨1; 俞世强1; 裴建明2; 赵玉峰2; 郑奇军1; 王伟光1
第四军医大学：1.西京医院心血管外科,2.生理学教研室，陕西 西安 710032
Author(s):
CAO Yu-kun1;  YU Shi-qiang1;  PEI Jian-ming2;  ZHAO Yu-feng2;  ZHENG Qi-jun1;  WANG Wei-guang1
1.Department of Cardiovascular Surgery, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
促生长激素释放多肽; 血压; 心肌收缩; 大鼠
Keywords:
ghrelin;  blood pressure;  left ventricular index
分类号:
R443.5　
DOI:
-
文献标识码:
A
摘要:
目的: 探讨促生长激素释放多肽(ghrelin,GRL)对大鼠心血管功能的影响及其意义。方法: 将40只成年雄性SD大鼠分为对照组和4个剂量的GRL组，静脉注射GRL后测量血流动力学指标。随后采用单细胞动态边缘检测系统测定细胞收缩功能的变化。结果: ①GRL各组平均动脉血压（MABP）较对照组明显下降（P<0.05）。②GRL各组单个心肌细胞收缩功能指标最大收缩幅度（PTA）、最大缩短和复长速率（±dL/dtmax）较对照组明显降低（P<0.05）。结论: GRL可剂量依赖性地降低大鼠的MABP，降低心肌细胞的收缩功能。
Abstract:
AIM: To investigate the effect of ghrelin on the cardiovascular system. METHODS: 40 male Sprague-Dawley (SD) rats were randomized into control group and four drug groups with five rats in each group. After the rats were anesthetized, a microcatheter was inserted into the left ventricle through the right carotid artery, and left ventricular developed pressure (LVDP), maximal velocity of left ventricular pressure development and decline (+LV dP/dtmax and -LV dp/dtmax) and heart rate (HR) were recorded by a biological signal collecting system. Simultaneously, mean arterial blood pressure (MABP) was measured by right femoral artery intubation. The rats were given either ghrelin (1×10-7-1×10-4 g/kg) or saline and MABP and left ventricular indexes were observed for 30 min. Contractile function of the myocytes were detected. RESULTS: No obvious changes of MABP were observed in control group after injection of saline. Intravenous (i.v.) injection of ghrelin decreased MABP in a dose-dependent manner by 11.4%, 15.8% and 27.1%, respectively, in 10-6 g/kg, 10-5 g/kg and 10-4 g/kg groups. No significant difference was observed in LVDP, +dp/dtmax, -dp/dtmax and HR 30 min after i.v. injection of ghrelin. The PTA ±dL/dtmax were significancly decreased in the GRL groups compared with the control group. CONCLUSION: Ghrelin decreases MABP of rats and contractile function of ventricular myocytes in a dose-dependent manner.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-11-06.通讯作者：俞世强，主任医师，主要从事微创心脏外科方面研究Email:yushiq@fmmu.edu.cn 共同通讯作者：裴建明，教授，主要从事心肌保护方面研究Email:jmpei@fmmu.edu.cn 作者简介：曹钰琨，硕士生Email:caoyuk@fmmu.edu.cn
更新日期/Last Update: 2010-06-22