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|本期目录/Table of Contents|

老年大鼠血管功能及能量代谢改变及机制

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
177-180
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Study on the vascular function and changes of the energy metabolism and mechanism in aged rats
作者:
王利; 吕安林; 赵晓梅; 邢玉洁; 燕学波; 赵伟
第四军医大学附属西京医院心血管内科，陕西 西安 710032
Author(s):
WANG Li;  Lv An-lin;  ZHAO Xiao-mei;  XING Yu-jie;  YAN Xue-bo;  ZHAO Wei
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
老年; 血管功能; 能量代谢; 腺苷酸活化蛋白激酶; 线粒体; 大鼠
Keywords:
aging;  vascular function;  energy metabolism;  AMPK;  mitochondria;  rat
分类号:
R617
DOI:
-
文献标识码:
A
摘要:
目的: 探讨老年大鼠血管功能的特点及其能量代谢和线粒体功能变化。方法： 将16只大鼠分为两组（n=8），即老年大鼠组和成年大鼠组。禁食12 h后，测量大鼠全血血糖水平和血浆胰岛素的水平。取大鼠胸主动脉制作成2~3 mm的血管环，连接到离体血管灌流及记录系统记录血管的舒张变化情况。取血管灌流液测定NO的含量。用Western blot法测量腺苷酸活化蛋白激酶（AMPKα1）的蛋白的表达。采用782型氧气消耗仪测定胸主动脉细胞内线粒体的氧气消耗。结果： 禁食12 h后，老年大鼠组全血血糖水平高于成年大鼠组［(5.70±0.03) vs.(4.21±0.06)mmol/L，P<0.05］。老年大鼠组血浆胰岛素水平高于成年大鼠组［(23.6±0.2) vs.(14.4±0.2)μU/ml，P<0.05］。老年大鼠胸主动脉舒张率下降显著［(0.41±0.01) vs.(0.79±0.01)%，P<0.05］。老年大鼠组NO的含量显著低于成年大鼠组 ［(9.07±0.15) vs.(45.33±0.38)μmol/L，P<0.05］。老年大鼠组AMPKα1的蛋白表达显著低于成年大鼠组（P<0.05）；线粒体氧气消耗，两组差异明显［(1.33±0.33) vs.(2.42±0.03)μmol/min/g，P<0.05］。结论： 老年大鼠组存在全身性胰岛素抵抗；血管舒张功能下降程度显著；AMPKα1蛋白的表达及线粒体氧气消耗能力下降，是血管功能下降的机制之一。
Abstract:
AIM: To explore the characteristics of vascular function and energy metabolism and mitochondrial function change in aged rats. METHODS: Sixteen rats were divided into two groups (n=8), namely, aged rat group and adult rat group. After 12 h of fasting, glucose and plasma insulin levels were tested. Aortic vascular rings ~2-3 mm were produced and connected to the aortic perfusion and recording system to record the diastolic changes. Blood vessel perfusate was taken to measure NO content. Western blot was used to measure the expression of AMP-activated protein kinase (AMPKα1) using 782-type oxygen consumption apparatus to measure the oxygen consumption of mitochondria. RESULTS: After 12 h of fasting, blood glucose and plasma insulin levels, were higher in aged rat group ［(5.70±0.03) vs.(4.21±0.06)mmol/L, P<0.05］, ［(23.6±0.2) vs.(14.4±0.2)μU/ml, P<0.05］. Diastolic function in aged group decreased significantly ［(0.41±0.01) vs.(0.79±0.01)%, P<0.059). For NO content, there was a significant difference between the aged rat group and adult rat group ［(9.07±0.15) vs.(45.33±0.38) μmol/L, P<0.05］. The expression of AMPKα1 in the aged rat group decreased significantly (P<0.05). For oxygen consumption in mitochondria there was a significant difference between the two groups ［(1.33±0.33) vs.(2.42±0.03) μmol/min/g, P<0.05). CONCLUSION: For the aged rat group, there was a systemic insulin resistance. Vascular diastolic function decreased significantly. AMPKα1 expression and mitochondrial oxygen consumption capacity decreased markedly. It may be one of the mechanisms for decrease of vascular function.

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备注/Memo

备注/Memo:
收稿日期：2010-04-22.基金项目:西京医院新技术研究基金项目资助（XJGX0510M08） 通讯作者:吕安林,副主任医师,主要从事冠心病的介入治疗Email：lvanlin@fmmu.edu.cn 作者简介:王利,硕士生Email:shenglidongjie288@163.com
更新日期/Last Update: 2010-12-10