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普罗布考抑制大鼠颈动脉球囊损伤后狭窄及机制

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
181-183,188
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Effects and mechanisms of probucol-inhibiting stenosis after balloon injury of carotid artery in rats
作者:
王四坤1; 黄晴2; 胡晓军1; 陈苏1
湖北省新华医院：1.心内科；2.心功能室,湖北 武汉 430015
Author(s):
WANG Si-kun1;  HUANG Qing2;  HU Xiao-jun1;  CHEN Su1
1.Department of Cardiology, 2.Department of Cardiac Function, Xinhua Hospital, Wuhan 430015, Hubei, China
关键词:
普罗布考; 内膜增生; 再狭窄
Keywords:
probucol;  intimal hyperplasia;  restenosis
分类号:
R543.4　
DOI:
-
文献标识码:
A
摘要:
目的: 观察普罗布考对大鼠颈动脉球囊损伤后狭窄的抑制作用，探索其可能机制。 方法： 随机将45只SD大鼠分为假手术组（n=15）、对照组（n=15）和治疗组（n=15），对照组及治疗组均用球囊损伤左颈总动脉，治疗组给予普罗布考灌胃。术后7、14、35 d处死大鼠，放免法检测大鼠血浆MDA，SOD水平；伊文思蓝染色观察血管内皮修复；PCNA染色检测细胞增殖；Image-Pro plus系统进行血管定量组织形态学分析。结果： 普罗布考明显升高血浆SOD水平，降低MDA水平；伊文思蓝染色结果显示普罗布考抑制内膜增生同时并未影响血管内皮化；治疗后14 d， 治疗组PCNA阳性细胞指数较对照组明显降低（n=15,P<0.01），内膜及中膜平滑肌细胞的抑制率为76％和65％，差异有统计学意义（P<0.05）；治疗后35 d,与对照组比较，治疗组降低内膜/中膜面积比达62.63%。结论： 普罗布考能升高血浆SOD水平、降低MDA水平，明显抗氧化，抑制血管平滑肌细胞异常增殖和新生内膜的形成，预防大鼠颈动脉球囊损伤后再狭窄。
Abstract:
AIM: To investigate the effects of probucol on intimal hyperplasia after balloon injury of carotid artery in rats and the impossible mechanism. METHODS: Forty-five male SD rats were randomly divided into sham-operated group, control group and therapy group. Rat left common carotid arteries were subjected to balloon injury in the control group and therapy group and probucol was administered IG to the therapy group. Arteries of the rats were harvested at 7, 14 and 35 days after balloon injury for immunohistochemical staining and H/E staining. Plasma MDA and SOD levels were detected by radioimmunoassay. Evans blue staining was used to measure the reendothelialized area and immunostaining of proliferating cell nuclear antigen (PCNA) was used to confirm the hyperplastic cells. Neointima thickening was assessed by intima to media area ratio (I/M) using a computer-based Image-Pro plus morphometric system. RESULTS: Compared with those in control group, MDA content decreased and SOD activity increased obviously in the therapy group. Probucol did not inhibit the reendothelialization in the injured carotid arteries as determined by Evans blue staining. PCNA-positive cell index in the intima and media at day 14 after therapy was markedly reduced in the therapy group compared with that in the control group. The area of intima and intima to media area ratio (I/M) on day 35 after balloon angioplasty was markedly attenuated in the therapy group compared with that in control group（0.74±0.11 vs. 1.98±0.53, P<0.01). Probucol reduced I/M by 62.63%. CONCLUSION: Probucol can increase plasma SOD level and reduce MDA level and inhibit vascular smooth muscle cell proliferation and neointimal formation after balloon injury of carotid artery, thus preventing restenosis after angioplasty in rats.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-05-02.作者简介：王四坤，硕士生Email：wangsikun135@sina.com
更新日期/Last Update: 2010-12-10