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胡椒碱对H2O2致兔心房肌细胞动作电位时程及L型钙电流异常的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
141-145
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Protective effect of piperine on abnormalities of action potential duration and L-type calcium current induced by hydrogen peroxide in rabbit atrial myocytes
作者:
刘 岩1; 李 泱2; 林 琨1; 田 苗1; 王玉堂1; 单兆亮1
(解放军总医院：1.心内科，2.老年心血管病研究所，北京 100853)
Author(s):
LIU Yan1;  LI Yang2;  LIN Kun1;  TIAN Miao1;  WANG Yu-tang1;  SHAN Zhao-liang1
(1.Department of Cardiology, 2.Institute of Geriatric Cardiology, PLA General Hospital, Beijing 100853, China)
关键词:
胡椒碱; 过氧化氢; 氧化应激; 动作电位时程; L型钙电流
Keywords:
piperine;  hydrogen peroxide;  oxidative stress;  action potential duration;  L-type calcium current
分类号:
R282.71
DOI:
-
文献标识码:
A
摘要:
目的:研究胡椒碱(PIP)对H2O2引起的单个兔心房肌细胞动作电位时程（APD）及L型钙电流(ICa,L)异常的保护作用。方法：采用全细胞膜片钳技术，观察10和50 μmol/L的H2O2引起单个兔心房肌细胞APD及ICa,L的改变，以及预先应用7 μmol/L的PIP对其的作用。结果：7 μmol/L的PIP对正常兔心房肌细胞APD、ICa,L及L型钙通道动力学无明显影响。在10和50 μmol/L的H2O2作用下，兔心房肌细胞APD50和APD90明显缩短(P<0.05)，静息膜电位（RMP）绝对值显著下降(P<0.05)，ICa,L峰值由(39.3±5.4) pA/pF降低至(32.8±2.0) pA/pF(P<0.05)，电流-电压曲线上移，通道稳态激活曲线右移，通道稳态失活曲线左移，但恢复时间不变。预先给予7 μmol/L的PIP可明显减轻H2O2对APD、ICa,L的抑制作用(P<0.01)，对L型钙通道动力学的异常影响。结论：PIP可减轻氧化应激对心房肌细胞APD、ICa,L的影响。
Abstract:
AIM:To study the protective effect of piperine on the abnormalities of action potential duration and L-type calcium current (ICa,L) induced by hydrogen peroxide in single rabbit atrial myocyte. METHODS: Whole-cell patch clamp technique was used to study the effect of H2O2 (10 and 50 μmol/L) on APD and ICa,L in single rabbit atrial myocyte and the effect of pretreatment with piperine (7 μmol/L). RESULTS: Piperine (7 μmol/L) had no significant effect on APD and ICa,L of normal single rabbit atrial myocyte as well as its channel dynamics. In the presence of 10 and 50 μmol/L H2O2, APD50 and APD90 decreased (P<0.05), amplitude of RMP disincreased (P<0.05), the peak of ICa,L was significantly reduced (P<0.05), I-V curve shifted upward, steady-state activation curve of ICa,L was shifted to positive potentials, and steady-state inactivation was shifted to negative potentials, but recovery from inactivation of ICa,L showed no remarkable changes. Piperine (7 μmol/L) significantly alleviated the inhibitory effect of H2O2 on APD and ICa,L (P<0.01) and protected the changes of ICa,L dynamics induced by H2O2. CONCLUSION: Piperine can alleviate abnormalities of APD and ICa,L induced by hydrogen peroxide in atrial myocytes.

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备注/Memo

备注/Memo:
收稿日期：2011-10-20.基金项目：国家自然科学基金项目资助（30772886） 通讯作者：单兆亮，主任医师，主要从事心脏电生理与起搏研究 Email：shanzl@sina.com 作者简介：刘岩，住院医师，硕士生 Email：3012003011@163.com
更新日期/Last Update: 2012-04-01