«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2012年第5期

页码:

583-587，590

栏目:

基础研究

出版日期:

2012-10-25

文章信息/Info

Title:

Observation of myocardial tissue injuries caused by a self-made, small animal quantitative trauma device

作者:

刘 宣¹; 吕广艳²; 吕国枫³; 于德钦¹; 陈小龙¹; 陈 冲¹; 李树壮¹; 3

(大连医科大学：1.生理学教研室，
2.中心实验室，
3.运动医学教研室，辽宁 大连 116044)

Author(s):

LIU Xuan¹;  LV Guang-yan²;  LV Guo-feng³;  YU De-qin¹;  CHEN Xiao-long¹;  CHEN chong¹;  LI Shu-zhuang¹; 3

(1.Department of Physiology; 2.Central Laboratory; 3.Department of Sports Medicine, Dalian Medical University, Dalian 116044, Liaoning, China)

关键词:

机械性损伤; 创伤仪器; 心肌; 大鼠

Keywords:

mechanical trauma;  device;  trauma device;  rat;  myocardium

分类号:

R542.2

DOI:

-

文献标识码:

A

摘要:

目的:观察使用自制小动物定量创伤仪，造成的大鼠全身机械性创伤。方法： 首先设计并组装制造实验用小动物定量全身性机械性创伤仪。将40 只大鼠分为对照组和创伤组，每组20只(n=20)，以40 r/min的转速旋转200圈,分别给予创伤组动物定量创伤，观察心肌组织中心肌细胞的凋亡指数、Caspase-3、iNOS、NO、NADPH氧化酶以及超氧阴离子（·O-2）的表达量，对照组未做任何处理。结果： 创伤组心肌细胞的凋亡指数、Caspase-3以及iNOS的表达、NO的产生、NADPH氧化酶的表达及·O-2的生成，均明显高于对照组（P<0.01)。结论： 使用自制小动物创伤仪，定量给予大鼠全身性机械性创伤，可造成大鼠心肌细胞发生明显的氧化应激反应和凋亡。

Abstract:

AIM:To observe myocardial tissue injury caused by a self-made, small animal quantitative trauma device. METHODS: Forty rats were randomly divided equally into two groups: control group (n=20) and trauma group (n=20). Rats of the trauma group were anesthetized with pentobarbital sodium (40 mg/kg), individually placed on a self-made small animal quantitative trauma device and were traumatized as the wheel rotated (200 revolutions (r) at a rate of 40 r/min). Measures were taken to observe the pathological changes of myocardial tissue. RESULTS: Compared with those in control group, the apoptotic index of the myocardial cells was elevated (P<0.01). Activity of caspase-3, expression of both iNOS and NADPH oxidase, and production of both NO and ·O-2 (superoxide anion) were also increased in the trauma group (all P<0.01). CONCLUSION: Mechanical trauma may induce significant oxidative stress and apoptosis in rat myocardial tissues by using a self-made, small animal quantitative trauma device.

参考文献/References

[1]Mac Donald CL,Johnson AM,Cooper D,et al.Detection of blast-related traumatic brain injury in U.S. military personnel[J].N Engl J Med,2011,364(22):2091-2100.
[2]Vasudevan AR,Kabinoff GS,Keltz TN,et al.Blunt chest trauma producing acute myocardial infarction in a rugby player[J].Lancet,2003,362(9381):370.
[3]谭源福,李耀华,林友俊,等.分级机械脑损伤动物模型的建立[J].中华创伤杂志,2000,16(2):100-102.
[4]蒋建新,粟永萍,黄跃生,等.严重创伤后早期全身性损害基础研究的现状与展望[J].中华创伤杂志,2002,18(4):197-199.
[5]Li SZ,Tao L,Jiao X,et al.TNFalpha-initiated oxidative/nitrative stress mediates cardiomyocyte apoptosis in traumatic animals[J].Apoptosis,2007,12(10):1795-1802.
[6]Wencker D,Chandra M,Nguyen K,et al.A mechanistic role for cardiac myocyte apoptosis in heart failure[J].J Clin Invest,2003,111(10):1497-1504.
[7]Li SZ,Jiao X,Tao L,et al.Tumor necrosis factor-alpha in mechanic trauma plasma mediates cardiomyocyte apoptosis[J].Am J Physiol Heart Circ Physiol,2007,293(3):H1847-H1852.
[8]Azad N,Iyer A,Vallyathan V,et al.Role of oxidative/nitrosative stress-mediated Bcl-2 regulation in apoptosis and malignant transformation[J].Ann N Y Acad Sci,2010,1203:1-6.
[9]Ascenzi P,di Masi A,Sciorati C,et al.Peroxynitrite-An ugly biofactor?[J].Biofactors,2010,36(4):264-273.
[10]Lu,S,Fan Z,Xu W,et al.L-cysteine attenuates peroxynitrite-elicited cytotoxicity to spiral ganglion neurons: possible relation to hearing loss[J].Neurol Res,2011,33(9):935-941.
[11]Mòdol T,Natal C,Pérez de Obanos MP,et al.Apoptosis of hepatic stellate cells mediated by specific protein nitration[J].Biochem Pharmacol,2011,81(3):451-458.
[12]Yu D,Neeley WL,Pritchard CD,et al.Blockade of peroxynitrite-induced neural stem cell death in the acutely injured spinal cord by drug-releasing polymer[J].Stem Cells,2009,27(5):1212-1222.
[13]Liu Y,Terata K,Chai Q,et al.Peroxynitrite inhibits Ca2+-activated K+ channel activity in smooth muscle of human coronary arterioles[J].Circ Res,2002,91(11):1070-1076.
[14]Bajt ML,Ramachandran A,Yan HM,et al.Apoptosis-inducing factor modulates mitochondrial oxidant stress in acetaminophen hepatotoxicity[J].Toxicol Sci,2011,122(2):598-605.
[15]Yang F,Wu W,Cao L,et al.Pathways of macrophage apoptosis within the interface membrane in aseptic loosening of prostheses[J].Biomaterials,2011,32(35):9159-9167.

备注/Memo

备注/Memo:

收稿日期：2012-06-08.
基金项目：国家自然科学基金项目资助（30971065）；辽宁省教育厅基金（2009A194）；大连市科技计划项目（2012E12SF074）
通讯作者：李树壮，教授，主要从事心血管损伤与保护的研究Email：shuzhuangli@126.com
作者简介：刘宣，硕士生Emai：liuxuan10102458@126.com

常用功能

更新日期/Last Update: 2012-11-16