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|本期目录/Table of Contents|

MicroRNA-195与Bcl-2/Bax在钙化大鼠主动脉中的表达及其相互作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第1期
页码:
20-024
栏目:
基础研究
出版日期:
2015-09-15

文章信息/Info

Title:
Expression and relationship of microRNA-195 and Bcl-2/Bax in rat aortic calcification
作者:
潘 莹; 曾宪钦; 段 雯; 张志远; 张文斌
（深圳市龙岗区人民医院心血管内科，广东 深圳 518172）
Author(s):
PAN Ying;  ZENG Xian-qin;  DUAN Wen;  ZHANG Zhi-yuan;  ZHANG Wen-bin
(Department of Cardiovascular Diseases, Longgang District People’s Hospital, Shenzhen 518172, Guangdong, China)
关键词:
Bcl-2/Bax; microRNA-195; 凋亡; 血管钙化; 大鼠
Keywords:
Bcl-2/Bax;  microRNA-195;  apotosis;  vascular calcification;  rat
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 观察Bcl-2/Bax和microRNA(miRNA)-195在大鼠血管钙化中表达的变化和相互关系。方法 将16只实验大鼠随机分对照组和钙化组，每组8只。钙化组肌肉注射维生素D3（30万U/kg，1次）和尼古丁灌胃（25 mg/kg溶于花生油中，早、晚各1次）诱导大鼠血管钙化模型，对照组大鼠予灌服等量生理盐水。建模6周后，股动脉放血处死大鼠，将主动脉用Von Kossa梁色后，观察其形态特征。用免疫组化染色法和免疫印迹法检测凋亡相关蛋白Bcl-2、Bax的表达，应用实时荧光定量多聚酶链式反应（qRT-PCR）法检测miRNA-195的表达。结果 钙化组的主动脉有大量黑色颗粒沉淀。钙化组主动脉组织中Bax的表达与对照组无明显差别，而Bcl-2的表达升高(P<0.05)。钙化组miRNA-195的表达量高于对照组（P<0.01）。Pearson直线相关分析显示，钙化的主动脉中miRNA-195表达的水平与Bcl-2表达的水平呈显著正相关(r=0.791，P<0.05)。结论 钙化的主动脉组织中miRNA-195的表达升高，并伴有Bcl-2蛋白的表达上调。miRNA-195与Bcl-2可能参与主动脉钙化。
Abstract:
AIM To observe the changes of microRNA195 and Bcl-2/Bax protein in rat aortic calcification. METHODSRats were randomly divided into two groups: control group (n=8) and calcification group (n=8). Rats in the calcification group received a single-dose I.M. injection of vitamin D3 (300 000 U/kg) in the morning and two doses of nicotine (25 mg/kg dissolved in peanut oil) by gavage, one in the morning and one in the evening to induce vascular calcification. Rats in the control group were treated with the same amount of normal saline. After a 6-week intervention, morphologic changes of arteries were examined by Von Kossa staining, expressions of Bcl-2/Bax were detected by immunohistochemistry and confirmed by Western blot, and expression of miRNA-195 was measured by quantitative real-time PCR (qRT-PCR). RESULTSMass black granules were seen deposited in the calcified vascular vessels in calcification group. No obvious difference was found in expression of Bax, but the expression of Bcl-2 protein was significantly upregulated in the calcification group (P<0.05). The level of miRNA-195 in the calcification group was significantly higher than in the control group (P<0.01). A positive correlation between the levels of miRNA-195 and Bcl-2 protein was observed (r=0.791, P<0.05). CONCLUSIONSLevel of miRNA-195 is upregulated along with the increasing expression of Bcl-2 protein in vascular calcification. miRNA-195 and Bcl-2 protein may be involved in vascular calcification.

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备注/Memo

备注/Memo:
收稿日期：2014-11-08.
作者简介：潘莹，主治医师，硕士 Email：nacypanying@163.com
更新日期/Last Update: 2015-09-16