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|本期目录/Table of Contents|

转录因子MCPIP在大鼠动脉粥样硬化病变进程中的表达

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第1期
页码:
25-028
栏目:
基础研究
出版日期:
2015-09-15

文章信息/Info

Title:
Expression of transcription factor MCPIP in atherosclerotic lesions in rats
作者:
罗 洁1; 2; 钱民章1
（1.遵义医学院生物化学教研室，贵州 遵义 563003；
2.贵州省第二人民医院检验科，贵州 贵阳 550004）
Author(s):
LUO Jie1; 2;  QIAN Min-zhang1
(1.Department of Biological Chemistry, Zunyi Medical College, Zunyi 563003, Guizhou, China；
2.Department of Laboratory, Second People's Hospital, Guiyang 550004, Guizhou, China)
关键词:
动脉粥样硬化; 单核细胞趋化蛋白-1诱导蛋白; 基因表达; 胸主动脉
Keywords:
atherosclerotic;  monocyte chemotactic protein-induced protein;  gene expression;  thoracic aorta
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的 观察转录因子MCPIP在大鼠动脉粥样硬化（AS）病变过程中的表达。方法 将60 只SD大鼠随机分为4个模型组和4个空白组；用喂饲高脂饲料加维生素D3灌胃的方法建立大鼠AS模型：分别建模2、4、6和7周，每一模型组设置相应空白组，喂饲普通饲料；HE染色观察各组大鼠胸主动脉病理改变；Real Time-PCR检测各组血管中MCPIP mRNA转录量；免疫组化检测各组血管中MCPIP蛋白表达量。结果 病理结果显示建模2周后，大鼠血管即出现AS病变，随着建模时间的延长，AS病变逐渐加重；Real Time-PCR结果显示，随着建模时间延长，各模型组血管中MCPIP mRNA的表达呈上升趋势；4个模型组与同期空白组比较，MCPIP mRNA的表达均有显著升高，差异明显（P<0.05或P<0.01）；免疫组化结果显示：随着建模时间延长，MCPIP 蛋白表达量也呈上升趋势；4个模型组MCPIP蛋白表达量均高于同期空白组，有显著差异（P<0.05或P<0.01）。结论 随着AS大鼠模型建模时间延长， 胸主动脉AS病变逐渐加重，血管中MCPIP的mRNA及蛋白表达量也呈递增变化，提示 MCPIP在 AS病变发生发展中有重要作用。
Abstract:
AIM To investigate the expression of monocyte chemotactic protein-induced protein (MCPIP) in the process of atherosclerosis in rats. METHODSSixty Sprague Dawley rats were randomly divided into four model groups and four control groups. Atherosclerosis was established by feeding rats with a high-fat diet and gavage of vitamin D3 for 2, 4, 6 and 7 weeks, respectively. Control rats were fed with a normal diet. The pathological changes of thoracic aorta were observed by HE staining. mRNA levels of MCPIP in thoracic aorta were determined by real time-PCR and MCPIP protein was detected by immunohistochemistry. RESULTSPathological examination showed that atherosclerotic lesions occurred 2 weeks after treatment and progressed with time. Real-time PCR results showed that vascular MCPIP mRNA increased in model rats compared with that in controls (P<0.05 or P<0.01). Immunohistochemistry showed increased expression of MCPIP protein with time. Expression of MCPIP protein in four model groups were significantly higher than in the corresponding normal control groups (P<0.05 or P<0.01). CONCLUSIONWe successfully established atherosclerosis in rats by feeding a high-fat diet plus vitami D3. Thoracic aortic atherosclerotic lesions occur 2 weeks after treatment and gradually are aggravated. Expressions of vascular MCPIP mRNA and protein increase after treatment, suggesting that MCPIP plays an important role in the development of atherosclerotic lesions.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2014-10-23.
>基金项目：遵义医学院2013年招标课题资助(F-613)
通讯作者：钱民章，教授，主要从事心血管分子生物学研究 Email:qian_mzh@hotmail.com
作者简介：罗洁，主管技师，硕士生 Email:luojie0511040108@126.com
更新日期/Last Update: 2015-09-16