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|本期目录/Table of Contents|

Inpp5f抑制血管紧张素Ⅱ诱导的心肌细胞肥大反应

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第2期
页码:
145-148
栏目:
基础研究
出版日期:
2015-11-25

文章信息/Info

Title:
Inpp5f attenuates angiotensin II-induced cardiomyocyte hypertrophy
作者:
柏丹娜1; 2; 高 延2; 王海昌1; 杨国栋3; 袁丽君4
(第四军医大学：1.西京医院心血管内科，3.生物化学与分子生物学教研室，4.唐都医院超声诊断科，陕西 西安 710032；
2.解放军第323医院心内科，陕西 西安 710054)
Author(s):
BAI Dan-na1; 2;  GAO Yan2;  WANG Hai-chang1;  YANG Guo-dong3;  YUAN Li-jun4
(1.Department of Cardiology, Xijing Hospital, 3.Department of Biochemistry and Molecular Biology, 4.Department of Ultrasound, Tangdu Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China；
2.Department of Cardiology, PLA 323 Hospital, Xi’an 710054, Shaanxi, China)
关键词:
磷酸酶; Inpp5f; 心肌肥大; 血管紧张素Ⅱ
Keywords:
phosphatase;  inositol polyphosphate-5-phosphatase F;  myocardial hypertrophy;  angiotensin II
分类号:
Q78
DOI:
-
文献标识码:
A
摘要:
目的 探讨磷酸酶Inpp5f在血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大反应中的作用。方法 用AngⅡ(1×10-7 mol/L)诱导大鼠心肌细胞H9c2发生肥大反应，qRT-PCR 和Western blot分别检测Myh7、Myh6和Inpp5f的表达变化；构建Inpp5f 过表达载体，给予细胞过表达外源性Inpp5f后，在AngⅡ诱导的心肌肥大反应中通过qRT-PCR 和Western blot查看Myh7、Myh6的表达变化。结果 在心肌细胞肥大反应中，Inpp5f表达降低。与对照相比，外源性过表达Inpp5f能显著减少AngⅡ引起的Myh7表达升高和Myh6表达降低。结论 在心肌肥大反应中，Inpp5f表达降低，而增加Inpp5f的表达能抑制心肌肥大反应，提示其作为心肌肥大保护因子的可能。
Abstract:
AIM To investigate the effect of inositol polyphosphate-5-phosphatase F (Inpp5f) on angiotensin II-induced cardiomyocyte hypertrophy. METHODS Cardiomyocyte hypertrophy was induced by angiotensin II (1×10-7 mol/L) in H9c2 cells. Expressions of Myh7, Myh6 and Inpp5f were detected by Western blot and real-time PCR. An Inpp5f overexpression vector was successfully constructed and transfected into H9c2 cells. Transcripts for Myh6 and Myh7 were detected by real-time PCR. RESULTS mRNA and protein levels of Inpp5f significantly decreased in the hypertrophy group. Overexpression of Inpp5f reversed the angiotensin II-induced increase in mRNA expression level of Myh7 and decrease in mRNA expression level of Myh6. CONCLUSION Overexpression of Inpp5f attenuates angiotensin II-induced hypertrophy of H9c2 cardiomyocytes.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2014-11-24.
基金项目：国家自然科学基金项目资助（81101050）；陕西省自然科学基础研究项目资助（2014JM4182）
通讯作者：袁丽君，教授，主要从事心血管疾病分子超声诊断研究 Email：yuanlj@fmmu.edu.cn
作者简介：柏丹娜，博士生 Email：danabai@163.com
更新日期/Last Update: 2016-04-25