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|本期目录/Table of Contents|

SHR和WKY大鼠血浆vasostatin-2水平的比较研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第5期
页码:
523-525,530
栏目:
基础研究
出版日期:
2016-04-25

文章信息/Info

Title:
Changes of circulating plasma vasostatin-2 levels and their significance in spontaneously hypertensive rats
作者:
陈长源; 康 宏; 赵存瑞; 牛 欢; 乙成成; 张 钲
（兰州大学第一医院心脏中心，甘肃 兰州 730000）
Author(s):
CHEN Chang-yuan;  KANG Hong;  ZHAO Cun-rui;  NIU Huan;  YI Cheng-cheng;  ZHANG Zheng
(Department of Cardiology, First Hospital, Lanzhou University, Lanzhou 730000, Gansu, China)
关键词:
vasostatin-2; 嗜铬粒蛋白A; 高血压
Keywords:
vasostatin-2;  chromogranin A;  hypertension
分类号:
R544.1
DOI:
-
文献标识码:
A
摘要:
目的 探讨自发性高血压（SHR）大鼠外周血vasostatin（VS）-2水平变化及阐明其意义。方法 采用ELISA方法检测14只SHR大鼠组和14只京都Wistar（WKY）大鼠组空腹外周血血浆VS-2水平，并利用清醒自由大鼠血流动力学动态检测技术检测大鼠血压、心动周期及压力感受性反射敏感性。结果 ①与WKY对照组相比，SHR组大鼠空腹血浆VS-2水平升高〔(0.43±0.05) pg/ml vs.(0.34±0.05) pg/ml，P<0.01〕；②Pearson相关分析显示，收缩压（SBP）、舒张压（DBP）、平均动脉压（MAP）与VS-2水平呈正相关（r=0.781，P<0.05；r=0.822，P<0.05；r=0.918,P<0.01）；HR与VS-2水平无关（r=0.350，P>0.05）；BRS与VS-2水平呈负相关（r=-0.959,P<0.01）；进一步使用多元线性逐步回归分析显示，MAP及BRS是VS-2水平的独立影响因子（P<0.05）。结论 SHR大鼠空腹血浆VS-2水平高于WKY大鼠，表明VS-2可能在动脉血压的调节过程中发挥作用。
Abstract:
AIM To investigate the changes of fasting circulating plasma vasostatin (VS)-2 levels and their relationship with blood pressure in spontaneously hypertensive rats (SHR). METHODSFasting plasma total VS-2 levels were measured by ELISA in 14 SHR and 14 Wistar-Kyoto (WKY) rats. Blood pressure, heart rate (HR) and baroreflex sensitivities (BRS) were measured by continuous recording system used for conscious freely moving rats. RESULTSVS-2 levels were significantly higher in SHR group compared with those in WKY group ［(0.43±0.05) pg/ml vs.(0.34±0.05) pg/ml, P<0.01］. Systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MAP) were positively correlated with VS-2 levels (r=0.781, P<0.05; r=0.822, P<0.05; r=0.918, P<0.01). HR was not correlated with VS-2 levels (r=0.350, P>0.05). BRS was negatively correlated with VS-2 levels (r=-0.959, P<0.01). In a multiple regression model including SBP, DBP, MAP and BRS, MAP and BRS were independent variables of VS-2 levels (standardized coefficient=-0.021, P<0.05; standardized coefficient=0.195, P<0.05; respectively). CONCLUSIONFasting circulating plasma VS-2 levels in SHR are higher than those in WKY rats, suggesting VS-2 may play a role in the regulations of hypertension.

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备注/Memo

备注/Memo:
收稿日期：2015-06-09.
基金项目：兰州大学中央高校基本科研业务费专项资金资助（lzujbky-2013-218）
通讯作者：张钲，主任医师，主要从事冠心病基础和临床研究 Email:zhangccu@yahoo.com.cn
作者简介：陈长源，主治医师，博士 Email:chenchangyuan.75@163.com
更新日期/Last Update: 2016-04-13