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Title:: Rho kinase may mediate impaired vasoconstriction of renal artery induced by hindlimb unweighting in rats

作者:: 吕强¹; 李宝义²; 王忠超¹; 刘焕¹; 白云刚¹; 杨静¹; 马进¹; （1.第四军医大学航空航天生理学教研室，陕西西安 710032；
2.沈阳军区兴城疗养院特勤科，辽宁兴城 125105）

Author(s):: L? Qiang¹; LI Bao-yi²; WANG Zhong-chao¹; LIU Huan¹; BAI Yun-gang¹; YANG Jing¹; MA Jin¹; (1.Department of Aerospace Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China；
2.Xingcheng Sanatorium, Shengyang Military Area Command, Xingcheng 125105, Liaoning, China)

关键词:: 模拟失重; 肾动脉; 收缩反应; Rho激酶

Keywords:: simulated microgravity; renal artery; vasoconstriction; Rho kinase

分类号:: R852.22

DOI:: -

文献标识码:: A

摘要:: 目的观察模拟失重对大鼠肾动脉收缩功能的影响及Rho相关蛋白激酶(Rho-associated protein kinase，ROCK)与其关系。方法以大鼠尾部悬吊4周建立模拟失重模型，采用离体血管环功能实验检测大鼠肾动脉收缩反应变化，通过免疫蛋白印迹技术检测大鼠肾动脉ROCK蛋白的表达，采用ROCK活性试剂盒检测ROCK活性。结果模拟失重大鼠肾动脉苯肾上腺素（Phenylephrine,PE）与氯化钾(KCl)诱导的收缩反应均明显降低（P<0.05），ROCK特异性抑制剂 Y-27632显著缩小模拟失重大鼠与正常大鼠动脉收缩反应之间的差异（P<0.05）；蛋白印迹结果显示，模拟失重大鼠肾动脉ROCK蛋白表达增加（P<0.05）；ROCK活性检测结果显示，模拟失重大鼠肾动脉ROCK活性降低（P<0.05）。结论模拟失重大鼠肾动脉收缩反应明显降低，抑制ROCK的作用可减小模拟失重大鼠与正常大鼠肾动脉收缩功能的差异，ROCK活性改变可能在模拟失重大鼠肾动脉收缩功能降低中发挥一定作用。

Abstract:: AIM To investigate the relationship between impaired vasoconstriction of renal artery and altered Rho-associated protein kinase (ROCK) induced by simulated microgravity in rats. METHODSFour-week hindlimb unweighting (HU) rats were adopted as animal models. Isometric force was measured by isolated tissue bath with or without Y-27632, a specific ROCK inhibitor. Protein expression and activity of ROCK were examined by Western blot and ROCK activity assay. RESULTSCompared with those in the control group, phenylephrine (PE)- and KCl-induced contractions were significantly decreased in the HU group (P<0.05) and inhibition of ROCK with Y-27632 significantly reduced the decrement of vasoconstriction between the control group and the HU group (P<0.05). The protein expression of ROCK was up-regulated (P<0.05), whereas ROCK activity was decreased (P<0.05) in renal arteries of the HU group. CONCLUSIONRenal artery vasoconstriction is decreased under simulated microgravity. Inhibition of ROCK may diminish the vasoconstrictive difference between control and simulated microgravity rats. The reduced activity of ROCK may be an important mechanism accounting for the impaired vasoconstriction in renal arteries induced by simulated microgravity in rats.

参考文献/References

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Rho激酶可能介导模拟失重所致大鼠肾动脉收缩功能的变化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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