我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

纳米纤维仿生支架可诱导iPSC向心肌细胞分化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2018年第1期
页码:
35-039
栏目:
基础研究
出版日期:
2017-10-10

文章信息/Info

Title:
Nanofibrous bionic scaffolds promote cardiomyocyte differentiation of induced pluripotent stem cells
作者:
刘雄涛1; 陈 焱2; 曾 迪1; 李 军1; 廉 诚1; 郑强荪3
(1. 第四军医大学唐都医院心内科，陕西 西安 710038；2.解放军第401医院急诊科，山东 青岛 2660711；3.西安交通大学第二附属医院心内科，陕西 西安 710004)
Author(s):
LIU Xiong-tao1;  CHEN Yan2;  ZENG Di1;  LI Jun1;  LIAN Cheng1;  ZHENG Qiang-sun3
(1.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China; 2.Department of Emergency, PLA 401 Hospital, Qingdao 266071, Shandong, China; 3.Department of Cardiology, Second Affiliated Hospital, Xi’an Jia
关键词:
诱导多能干细胞; 纳米纤维支架; 细胞分化; 心肌细胞
Keywords:
induced pluripotent stem cell;  poly-(ε-caprolactone);  nanofibrous scaffold;  cardiomyocyte
分类号:
R3；Q2
DOI:
-
文献标识码:
A
摘要:
目的 利用诱导多能干细胞（induced pluripotent stem cell,iPSC）与纳米纤维仿生支架构建人工心肌，探讨纳米纤维支架用于诱导多能干细胞心肌特异性分化的可行性及潜在作用，为构建组织工程心肌提供参考。方法 采用静电纺丝技术制作聚己内酯/明胶复合纳米纤维仿生支架，接种鼠源Oct4-GFP+ iPSCs培养分化15 d。鼠源Oct4-GFP+ iPSCs同样接种于组织培养皿，培养分化15 d作为对照组。免疫荧光染色检测心肌细胞特异性标志物，流式细胞计数统计心肌细胞分化效率。结果 Oct4-GFP+ iPSCs能够在聚己内酯/明胶复合纳米纤维仿生支架上正常增殖、分化；培养15 d后，Oct4-GFP+ iPSCs在支架上分化出心肌细胞特异性标志物cTnT/MLC2a双染阳性的心肌细胞；而且支架组心肌细胞分化效率显著高于对照组（P<0.05）。结论 聚己内酯/明胶纳米纤维仿生支架支持iPSCs增殖，且能够促进其向心肌细胞分化，适用于组织工程心肌构建。
Abstract:
AIM To explore the viability and the potential role of bionic scaffolds on cardiomyocyte (CM) differentiation of induced pluripotent stem cells (iPSCs). METHODS Oct4-GFP+mouse iPSCs (iPSCs) were cultured on poly-(ε-caprolactone)/Gelatin (PCL/Gelatin) composite nanofibrous bionic scaffolds, which were fabricated by an electrospinning technique and were differentiated spontaneously for 15 days. Oct4-GFP+ mouse iPSCs (iPSCs) cultured on tissue culture plates (TCPs) were used as control. The development of CMs was verified by immunofluorescence staining of cardiac specific markers, such as cardiac Troponin T (cTnT) and myosin light chain 2a (MLC2a). The efficiency of iPSC-derived-CMs was calculated by flowcytometric analysis. RESULTS The results demonstrated that Oct4-GFP+iPSCs proliferated on the PCL/Gelatin composite nanofibrous scaffold and grew in colonies during the spontaneous differentiation period. Immunofluorescence staining after differentiation for 15 days revealed that some of the iPSCs-derived cells were co-labeled with cTnT and MLC2a. Moreover, flowcytometric analysis demonstrated that the ratio of cTnT+-cells derived from iPSCs was significantly higher in PCL/Gelatin scaffolds than in controls (P<0.05). CONCLUSION These results suggest that the PCL/Gelatin composite nanofibrous bionic scaffold could support the proliferation and cardiomyocyte differentiation of Oct4-GFP+ iPSC and could be potentially used as a platform for myocardium tissue engineering.

参考文献/References

[1]Shi Y,Inoue H,Wu JC,et al.br />
[2]Inoue H,Nagata N,Kurokawa H,et al.iPS cells:a game changer for future medicine[J].EMBO J,2014,33(5):409-417.

[3]Kochegarov A,Lemanski LF.New Trends in Heart Regeneration:A Review[J].J Stem Cells Regen Med,2016,12(2):61-68.

[4]李 天，邹远康，李子超，等.诱导多能干细胞与转分化在心肌再生中的研究进展[J].心脏杂志,2017,29(1):109-111.

[5]Reis LA,Chiu LL,Feric N,et al.Biomaterials in myocardial tissue engineering[J].J Tissue Eng Regen Med,2016,10(1):11-28.

[6]Gautam S,Dinda AK,Mishra NC.Fabrication and characterization of PCL/gelatin composite nanofibrous scaffold for tissue engineering applications by electrospinning method[J].Mater Sci Eng C Mater Biol Appl,2013,33(3):1228-1235.

[7]Kim PH,Cho JY.Myocardial tissue engineering using electrospun nanofiber composites[J].BMB Rep,2016,49(1):26-36.

[8] Sajkiewicz P,Kolbuk D.Electrospinning of gelatin for tissue engineering--molecular conformation as one of the overlooked problems[J].J Biomater Sci Polym Ed,2014,25(18):2009-2022.

[9]Happe CL,Engler AJ.Mechanical Forces Reshape Differentiation Cues That Guide Cardiomyogenesis[J].Circ Res,2016,118(2):296-310.

[10]Zeng D,Ou DB,Wei T,et al.Collagen/β(1)integrin interaction is required for embryoid body formation during cardiogenesis from murine induced pluripotent stem cells[J].BMC Cell Biol,2013,14:5.

备注/Memo

备注/Memo:
收稿日期：2017-03-16.基金项目：国家自然科学基金项目资助(31271039，31400832) 通讯作者：郑强荪，主任医师，主要从事心脏疾病临床研究 Email：zhengqiangsun@yeah.net 作者简介：刘雄涛，主治医师，博士 Email：liuxiongtaovip@163.com 共同第一作者：陈焱，主治医师，博士 Email：chenyen19147@163.com
更新日期/Last Update: 1900-01-01