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|本期目录/Table of Contents|

木犀草素减轻糖尿病小鼠心肌损伤及其机制

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2018年第1期
页码:
30-034,039
栏目:
基础研究
出版日期:
2017-10-10

文章信息/Info

Title:
Effects of luteolin on myocardial injury and underlying mechanisms in diabetic mice
作者:
王 雷1孙书红23付爱萍4王 曼1谢芳元1
(1.西安市第四医院心内科 陕西 西安 710004;2.武警陕西总队医院心内科 陕西 西安 710054;3.第四军医大学西京医院心内科 陕西 西安 710032;4.陕西航天医院内分泌科 陕西 西安 710025)
Author(s):
WANG Lei1 SUN Shu-hong23 FU Ai-ping4 WANG Man1 XIE Fang-yuan1
(1.Department of Cardiology, Fourth Hospital of Xi’an, Xi’an 710004, Shaanxi, China; 2.Department of Cardiology, Hospital of Shaanxi Armed Police Corps, Xi’an 710054, Shaanxi, China; 3.Department of Cardiology, Xijing Hospital, Fourth Military Medical Uni
关键词:
木犀草素糖尿病心肌病凋亡Rho/ROCK2信号通路
Keywords:
luteolin diabetic cardiomyopathy apoptosis RhoA/ROCK2 signaling
分类号:
R284.1
DOI:
-
文献标识码:
A
摘要:
目的 探讨木犀草素(Luteolin,LTL)对糖尿病小鼠心肌损伤影响及其可能调控机制。方法 腹腔注射链脲霉素制备1型糖尿病小鼠模型,将雄性C57/BL6J小鼠分为3组(每组16只):对照组,糖尿病组,糖尿病+木犀草素组。糖尿病+木犀草素组给予LTL 50 mg/(kg·d)灌胃,对照组和糖尿病组给予50 g/L的羧甲基纤维素钠灌胃。药物治疗3月后,小鼠心脏超声检测心功能;Masson评估胶原容积分数(collagen volume fraction,CVF);Tunel法检测心肌细胞凋亡;Western blot检测Rho和ROCK2的表达。结果 与对照组相比,糖尿病组各项心功能指标显著降低(P<0.05),CVF显著增高(P<0.05),小鼠心肌细胞凋亡显著增高(P<0.05),Rho和ROCK2的表达显著增加(P<0.05);与糖尿病组相比,糖尿病+木犀草素组各项心功能指标明显改善(P<0.05),CVF显著降低(P<0.05),小鼠心肌细胞凋亡显著降低(P<0.05),Rho和ROCK2的表达降低(P<0.05)。结论 LTL可能通过抑制RhoA/ROCK2信号通路减少糖尿病小鼠心肌细胞凋亡,从而减轻糖尿病心肌损伤。
Abstract:
AIM To explore the effects and mechanisms of luteolin on heart impairment in diabetic mice. METHODS Diabetes was induced by a single intraperitoneal of streptozotocin. Male C57/BL6J mice were randomly divided into three groups (n=16): control, diabetes, diabetes+luteolin. The diabetes+luteolin group were given a dose of 50 mg/(kg·d) of luteolin, while the control and diabetes groups received 50 g/L sodium carboxymethyl cellulose gavage. For 3 months, echocardiography was employed to evaluate cardiac function. Collagen volume fraction (CVF) was measured by masson staining and apoptotic index was examined by Tunel. Western blot was used to determine the expression of Rho and ROCK2. RESULTS Compared with the control group, cardiac function attenuated significantly (P<0.05), with increased CVF (P<0.05). There was enhanced apoptotic index (P<0.05) and expression of Rho and ROCK2 were upregulated (P<0.05) when compared to the control group. Compared with those in the diabetes group, the cardiac function was significantly improved (P<0.05) and CVF significantly diminished (P<0.05). There was a restored apoptotic index (P<0.05), and there was reduction of Rho and ROCK2 levels (P<0.05) by treatment with luteolin in the diabetes+luteolin group. CONCLUSION Luteolin significantly decreases the apoptotic index and protects against myocardial injury in diabetic mice, possibly by inhibiting activation of RhoA/ROCK2 signaling.

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备注/Memo

备注/Memo:
收稿日期:2017-01-10.通讯作者:谢芳元,主任医师,主要从事冠心病的防治研究 Email:xiefangyuan2004007@126.com 作者简介:王雷,主治医师,硕士 Email:497552148@qq.com
更新日期/Last Update: 1900-01-01