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|本期目录/Table of Contents|

131I放射自显影法检测活体心传导束(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
119-121
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Cheekout of vital heart conduction bundles by 131I autoradiography
作者:
程彦斌1;  王　晖1;  程殿臣2;  程殿威1;  邢华平3;  谢定雄1
1.兰州医学院人体解剖学教研室, 甘肃 兰州 730000; 2.兰州市妇幼保健医院麻醉科;3.兰州市城关区医院眼科
Author(s):
CHENG Yan-bin1; WANG Huei1;  CHENG Dian-chen2;  CHENG Dian-wei1;  XING Hua-ping3; XIE Ding-xong1
1.Department of Anatomy,Lanzhou Medical College,Lanzhou Gansu 730000, China
关键词:
心传导束;  染色;  放射自显影;  放射性核素;  电镜;  狗
Keywords:
heart conduction bundle;  staining;  autoradiogruphy;  radionuclide;  electron microscope; dogs
分类号:
R322.1;Q954.56
DOI:
-
文献标识码:
A
摘要:
目的 从细胞分子水平阐明碘仅使活体心传导组织显色而心肌组织不显色的内在机制.方法 选用5只健康家犬,手术暴露室间隔,用131I喷染左、右束支,使其显色,经取材、制片、放射自显影术后,用光、电观察.结果 ①左、右束支清晰可辨,略呈棕黑色,与邻近心肌区别明显.②组成心传导束的普肯野细胞(Purkinje cells)染色较淡,成族团状,周围包有结缔组织,位于心内膜和心肌层之间.胞核居中,染色更淡,细胞长径约20～80 μm,横径约20～60 μm.③Purkinje细胞内可见大量散在的显影银粒子,心肌细胞内未见显影银粒子.结论 131I通过Purkinje细胞膜进入到了细胞内,推测Purkinje细胞膜上可能存在某种转运机制,而心肌细胞膜上则没有.
Abstract:
AIM To study the mechanism of vital heart conduction bundle branches stained with radioiodine at cellular level. METHODS Five dogs were operated on to expose the ventricular septa from right and left side of the hearts and sprayed with 131 I,so that the left and right conduction bundle branches were stained. After sampling,manufacture of thin pieces and autoradiography,the Purkinje cell and myocardium cell structures were examined under light and electron microscopes. RESULTS ①The right and left bundle branches were stained very clearly with 131I;the colour of the bundle fibres were brown, but the adjacent myocardiums were not. ②The purk inje cells composing the heart conduction bundle branches were surrounded by connective tissues and located between the endocardium and the myocardium. The purk inje cell was lightly stained and the cell nucleus was located at the center. The Purk inje cell′s diameter was 20～ 80 μm in length and 20～ 60 μm in breath. ③There were a large number of marking silver particles in the Purk inje cell but there were not any in the myocardium. CONCLUSION 131I entered the Purk inje cells through the membrane but it did not enter the myocardium. There might be certain transport mechanism on the Purk inje cell membrane, there was no such mechanism on the myocardium membrane.

参考文献/References

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[2] 程彦斌. 辅酶Q10-I2 活体显示心脏传导束的实验研究[J]. 中华医学杂志, 1996, 76: 225- 227.

[3] Ishli K, Koga Y,Nakamara K, et al. Clinical application of a new vital staining method for the conduction system during heart operations[J]. Thorac Cardiovasc Surg , 1988, 95: 592- 594.

[4] Tamiya T. A study on the course of the atrioventricular conduction system in congenital heart disease electrophysio logical delineation and histologic investigation [J]. Thorac Surg , 1986, 40(6) : 599- 602.

[5] 刘鼎新. 放射自显影技术[M]. 上海: 科学出版社, 1986. 28.

[6] 杨抚华. 医学细胞生物学概论[M]. 四川: 四川科学技术出版社, 1996. 65.

[7] 黄方炯. ATP-I2 活体显示心脏传导束的实验研究[J]. 中华心血管外科杂志, 1990, 6 (3) : 161- 163.[8] 黄惠民, 刘锦纷, 余晓晴, 等. 心脏传导束染色的实验研究[J].中国临床解剖学杂志, 1995, 13: 225- 227.

备注/Memo

备注/Memo:
收稿日期：2001-07-05.
更新日期/Last Update: 2002-03-01