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一氧化氮对培养乳鼠心肌细胞低氧复氧损伤的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第2期
页码:
116-117,120
栏目:
基础研究
出版日期:
2004-03-01

文章信息/Info

Title:
Effect of nitric oxide on anoxia/reoxyge nation injuries of neonatal rat cardiomyocytes
作者:
高好考; 王海昌; 张荣庆; 李敬霞
第四军医大学西京医院心血管内科, 陕西 西安 710032
Author(s):
GAO Hao-kao; WANG Hai-chang; ZHANG Rong-qing; LI Jing-xia
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China
关键词:
一氧化氮; 心肌细胞; 缺氧/复氧; S-亚硝基-已酰青酶胺
Keywords:
nitric oxide;  cardiomyocytes;  anoxia/reoxygenation;  SNAP
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的:研究NO对乳鼠心肌细胞低氧/复氧(A/R)损伤的影响。方法:建立心肌细胞A/R损伤模型,随机分为6组:A组,正常对照组;B组,单纯低氧/复氧(A/R低氧2h,复氧1h);C组,低氧预处理,低氧20min后复氧20min,然后A/R;D、E、F组为NO预处理组,加入S-亚硝基-已酰青酶胺(SNAP)分别使其终浓度为0.5、1、2mmol/L,预处理40min后A/R。于复氧后测定各组培养液中肌酸激酶(CK)、蛋白漏出量的变化及细胞内丙二醛(MDA)含量和细胞存活率。结果:单纯低氧/复氧组和2mmol/LSNAP组CK、蛋白漏出量、MDA水平显著升高(P<0.01 vs 正常组),细胞存活率显著降低(P<0.01 vs 正常组)。0.5和1mmol/LSNAP预处理组和低氧预处理组上述变化明显减轻(P<0.01 vs 低氧复氧组)。结论:NO预处理对乳鼠心肌细胞A/R损伤具有保护作用,并具有浓度依赖性。
Abstract:
AIM:To study the effect of Nitric oxide(NO) on anoxia/reoxygenation(A/R) injuries f neonatal rat cardiomyocytes. METHODS: Ananoxia/reoxygenation model of myocardial cells of neonatal SD rats was established. The cells were randomly divided into six groups: control group(A): without any pretreatment; anoxia/reoxygenation(A/R) group (B): 2-hour anoxia followed by 1-hour reoxygenation; anoxia preconditioning group(C): 20-minute anoxia followed by 20-minute reoxygenation and then A/R; NO preconditioned group (D): adding s-nitroso-nacetyl-penicillamine (SNAP) into culture medium till the final concentration of 0.5mmol/L, after 40 minutes preconditioning and then A/R; NO preconditioned group (E): adding SNAP into culture medium till the fina lconcentration of 1mmol/L, after 40 minutes preconditioning and then A/R; NO preconditioned group (F):a dding SNAP into culture medium till the final concentration of 2 mmol/L,after 40 minutes' preconditioning and then A/R.The activity of creatine kinase (CK), the leakage of protein, the contents of cellular malondiadehyde (MDA), the rate of cell viability of each group were evaluated after reoxygenation. RESULTS: The anoxia/reoxygenation group and 2 mmol/L SNAP group showed agreatin creases in levels of CK, protein and MDA, and adecrease in cell viability (P< 0.01 vs group A).The anoxia preconditioned, the 0.5 mmol/L and the 1mmol/L SNAP groups significantly attenuated these change (P<0.01 vs group B). CONCLUSION: NO canexerta protective effect on A/R injury of neonatal rat cardiomyocytes inaconcentrati on dependent manner.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2003-9-8
更新日期/Last Update: 2004-03-01