VEGF对人血单核细胞基质金属蛋白酶表达及活性的影响X(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2004年第4期
- 页码:
- 308-311
- 栏目:
- 基础研究
- 出版日期:
- 2004-07-01
文章信息/Info
- Title:
- Effect of VEGF on expression and activity of MMPs of human monocytes
- Author(s):
- WANG Chang-qian; TANG Da-ming ; WANG Li-min; XIE Xiu-lan; XU Yi-min; DING Hong-yi; SONG Wei; WANG Bin-yao; HUANG Ding-jiu
- Department of Cardiology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China
- 分类号:
- 541. 4
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 探讨血管内皮生长因子(VEGF) 对人单核细胞源巨噬细胞(HMDM) 基质金属蛋白酶(MMPs) 的表达及其活性的影响。方法: 分别应用RT-PCR 和Western blot检测MMPs (MMP-2 和MMP-9)基因和蛋白表达,Zymography活性分析检测MMPs活性。结果: VEGF可明显增加人单核细胞源巨噬细胞(HMDM)MMP-2、MMP-9mRNA及其蛋白表达和活性。结论:VEGF可刺激HMDMMMP-2、MMP-9mRNA及其蛋白的表达, 增强其活性, 因而有可能促进动脉粥样硬化斑块内基质降解, 进而引起粥样硬化斑块破裂。
- Abstract:
- AIM: To observe the effect of VEGF on expression and activitiy of Matrix Metalloproteinase (MMPs). METHODS: The gene and protein expression of MMPs (MMP-2 and MMP-9) were respectively detected by RT-PCR and Western blot. MMPs’ activity was detected by Zymography. RESULTS: The expressions and activities of MMP-2 and MMP-9 mRNA of human monocytes were increased significantly by VEGF. CONCLUSION : As VEGF can stimulate the expressions and activities of MMP-2 and MMP-9 of human monocytes, it may increase the breakdown of matrix in lesions of atherosclerosis and enhance the lesions’vulnerability.
参考文献/References
[1] Xu XP, Meisel SR, Ong JM , et al. Oxidized low-density lipoprotein regulates matrix metalloproteinae2-and its tissue inhibitor in human monocyte-derived macrophages[J].Circulation, 1999, 99: 993- 998.
[2] Wang H, Keiser JA. Vascular endothelial growth factor upregulates the expression of matrix metalloproteinases in vascular smooth cells: role of flt-1 [J]. Circ Res, 1998, 83:832- 840.
[3] Galis ZS, Sukhova GK, Lark MW , et al. Increase expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques[J]. J Clin Invest, 1994, 94: 2493- 2503.
[4] Inoue M , Itoh H, Ueda M , et al. Vascular endothelial growth factor(VEGF) expression in human coronary atherosclerotic lesions: Possible pathophysiological significance of VEGF in progression of atherosclerosis[J]. Circulation, 1998, 98: 2108 - 2116.
[5] Cuici JA , Mao DL , Bohner DG, et al. Preoperative treatment with doxycycline reduces aortic wall expression and activation of matrix metalloproteinases in patients with abdominal aortic aneurysms[J]. J Vasc Surg , 2000, 31: 325- 342.
[6]Grant GM , Cobb JK, Castillo B, et al. Regulation of matrix metalloproteinases follows cellular transformation [J]. J Cell Physiol, 1996, 167 (1) : 177- 183.
[7]Falk E, Shah PK, Fuster V. Coronary plaque disruption [J].Circulation, 1995, 92: 657- 667.
[8] Galis ZS, Sukhova GK, Libby P. Microscopic localization of active proteinases by in situ zymography: detection of matrix metalloproteinase activity in vascular tissue [J]. FASEB J ,1995, 9: 974- 980.
[9]Murphy G, Willenbrock F, Crabbe T, et al. Regulation of matrix metalloproteinase activity [J]. Ann NY Acad Sci,1994, 732: 31- 41.
[10]Moreau M , Brocheriou I, Petit L , et al. Interleukin-8 mediates downregulation of tissue inhibitor of metalloproteinase-1 expression in cholesterol-loaded human macrophages:relevance to stability of athero sclerotic plaque[J]. Circulation,
1999, 99: 420- 426.
[11] Beck L Jr, D’Amore PA. Vascular development: cellular and molecular regulation[J]. FASEB J , 1997, 11: 365- 373.
[12] Wang H, Moore S, Alavi MZ. Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in rabbit aortic neointima after
selective de-endothelialization[J]. Atherosclerosis, 1996, 126:95- 104.
[13] Unemori EN , Ferrara N , Bauer EA , et al. Vascular endothelial growth factor induces interstitial collagenase
expression in human endothelial cells [J]. J Cell Physiol,1992, 153: 557- 562.
备注/Memo
- 备注/Memo:
- 收稿日期: 2003-05-23X基金项目：本课题受上海市青年科技启明星计划（98QB14014）资助
常用功能
统计/Statistics
- 摘要浏览/Viewed964
- 全文下载/Downloads786
- 评论/Comments
