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血管钠肽对人桡动脉的舒张作用及其机制(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
407-409,413
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Relaxation of human radial artery by vasonatrin peptide and its mechanism
作者:
于　军1; 朱妙章2; 韦耿泽2; 吕顺艳2; 郭海涛2; 周京军2; 陈宝莹3; 康云帆4
第四军医大学:1. 教学实验中心,2. 生理学教研室,3. 唐都医院放射科, 4. 西京医院心血管外科中心, 陕西西安710032
Author(s):
YU Jun1; ZHU Miao-zhang2; WEI Geng-ze2; Lv Shun-yan2; GUO Hai-tao2; ZHOU Jing-jun2; CHEN Bao-ying3; KANG Yun-fan4
1. Experiment Teaching Center , 2. Department of Physiology , 3. Radiology Department of Tangdu Hospital , 4. Cardiovascular Surgery Center of Xijing Hospital , Fourth Military Medical University , Xi’an , Shaanxi 710032 ,China
关键词:
血管钠肽; 人桡动脉;  cGMP; TEA; 美蓝
Keywords:
vasonatrin peptide ;  human radial artery ;  cGMP ;  tetraethylammonium;  methylene blue
分类号:
Q463 ;R33
DOI:
-
文献标识码:
A
摘要:
目的:研究血管钠肽(VNP) 对人桡动脉(human radial artery , HRA) 的舒张作用及其机制。方法:采用离体血管 灌流的方法,观察VNP 对内皮完整和去内皮HRA 的舒张作用,以及82Br2cGMP、NPR2A、B 选择性抑制剂HS214221、鸟 苷酸环化酶选择性抑制剂美蓝(methylene blue , MB) 和Ca2 + 激活K+ 通道(Ca2 +2activated K+ channel , KCa) 的选择性抑 制剂TEA 对这一过程的影响。结果:VNP(10 210～10 - 6 mol/ L) 可剂量依赖性地舒张HRA ,该作用无内皮依赖性。82 Br2cGMP(10 - 7～10 - 3mol/ L) 可模拟VNP 的血管舒张效应。HS214221 (2 × 10 - 5mol/ L) 或MB (10 - 5mol/ L) 完全阻断 VNP 舒张作用。TEA(10 - 3mol/ L) 减弱VNP 的舒血管作用。结论:VNP 对HRA 具有不依赖内皮的舒张作用,它主要 是通过作用于NPR2A、B ,即钠尿肽的鸟苷酸环化酶耦联受体,升高细胞内的cGMP 水平。KCa可能是其舒张作用的 重要效应分子。为应用VNP 防治血管移植术后的痉挛提供了重要的理论依据。
Abstract:
AIM: To investigate the vasorelaxing effect of vasonatrin peptide (VNP) on human radial artery (HRA) and to reveal its underlying mechanism. METHODS : The vasorelaxing effect of VNP on HRA with and without endothelium was observed by perfusion in vitro. Effects of 8-Br-cGMP , HS-142-1 , the selective inhibitor of NPR-A and B , methylene blue (MB) , the selective inhibitor of GC , and tetraethylammonium (TEA) , the selective inhibitor of Ca2 + -activated K+ channel (KCa) , were also investigated. RESULTS : It was found that VNP from 10 - 10to 10 - 6 mol/ L induced a concentration2 dependent relaxation of HRA , which was independent of the endothelium. This effect was mimicked by 82Br2cGMP (10 - 7～10 - 3mol/ L) , and was abolished by either HS-142-1 at 2 ×10 - 5mol/ L , or MB at 10 - 5mol/ L. It was also attenuated by TEA at 10 - 3 mol/ L. CONCLUSION: The present study demonstrated that vasorelaxing effect of VNP on HRA is independent of endothelium , It results from an increase in the intracellular cGMP level by the activation of natriuretic peptide guanyl cyclase receptors in VSMCs , and KCa is an important mediator. The study has provided new and useful information for the use of VNP in the prevention and treatment of the spasm of transplanted blood vessels.

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备注/Memo

备注/Memo:
收稿日期：2004-3-5
更新日期/Last Update: 2004-09-01