我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

内皮细胞生长状态与血管平滑肌细胞增殖迁移能力的关系(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第5期
页码:
403-406
栏目:
基础研究
出版日期:
2004-09-01

文章信息/Info

Title:
Relation between the endothelial cell growth state and the proliferation and migration properties of vascular smooth muscle cells
作者:
武晓静1; 黄　岚1; 赵　刚1; 周音频1; 崔　斌1; 周　骐2
第三军医大学新桥医院:1. 全军心血管内科中心, 2. 心外科, 重庆400037
Author(s):
WU Xiao-jing1; HUANG Lan1; ZHAO Gang1; ZHOU Yin-pin1; CUI Bin1; ZHOU Qi2
1. Cardiologic Center of PLA , 2. Department of Cardiovascular Surgery , Xinqiao Hospital , Third Military Medical University , Chongqing 400037 , China
关键词:
内皮细胞; 平滑肌细胞; 增殖; 迁移
Keywords:
endothelial cell ;  smooth muscle cell ; proliferation ;  migration
分类号:
Q418
DOI:
-
文献标识码:
A
摘要:
目的: 探讨内皮细胞生长状态与血管平滑肌细胞增殖迁移能力的关系。方法: 通过建立细胞共培养体系,检 测不同内皮生长状态对血管平滑肌细胞3H2TdR 掺入、增殖细胞核抗原(PCNA) 蛋白表达、细胞迁移计数和α2SM2actin mRNA 表达的影响。结果: 融合生长内皮使平滑肌细胞3H2TdR 掺入量和PCNA 蛋白表达明显降低,上调平滑肌细 胞α2SM2actin mRNA 表达;而对数生长内皮细胞使平滑肌细胞3H2TdR 掺入量和PCNA 蛋白表达明显升高,下调平滑 肌细胞α2SM2actin mRNA 表达。对照组平滑肌细胞在基础状态下存在少量迁移,对数增殖内皮细胞组平滑肌迁移 数比对照组增高约4 倍( P < 0. 05) ,而融合生长内皮细胞组平滑肌迁移数仅为对照组的0. 5 倍( P < 0. 05) 。结论: 内皮细胞生长状态不同,对平滑肌细胞生物学特性的影响也不同,增殖期内皮明显促进平滑肌细胞增殖迁移、下调 平滑肌细胞α2SM2actin mRNA 表达。
Abstract:
AIM: To investigate the relation between the altered endothelial cells ( EC) growth status and the proliferation and migration properties of vascular smooth muscle cells(SMCs) . METHODS: In the cell coculture system , ECs were seeded on the lower chamber and SMCs on the upper chamber. 　3H-TdR incoporation and PCNA protein expression were used to determine the effects of varied EC growth states on the proliferation of vascular SMCs. The number of SMCs migration was counted. RT-PCR was used to analyze the influence of varied EC growth states on the expression ofα-SM-actin mRNA. RESULTS : 　3H-TdR incorportation and the expression of PCNA protein were significantly lower in the confluent EC group and significantly higher in the proliferative EC group. The number of migrating cells was about 4 times higher in the proliferative EC group( n = 6 , P < 0. 05) than in the control group ,while that in the confluent EC group was only half of the number of the control ( n = 6 , P < 0. 05) . The proliferative ECs inhibited and confluent EC promoted significantly the expression ofα-SM-actin mRNA. CONCLUSION: These results suggest that the biologic features of SMCs are influenced by EC growth state. Proliferative ECs promoted significantly the proliferation and migration of SMCs and down-regulateα-SM-actin mRNA expression.

参考文献/References

[1 ] Reidy MA. In vivo proliferation of vascular smooth muscle cells in vessels with intact endothelial cover (Abstract) [J ] . Fed Proc ,1986 ,45 :683.

[2 ] Axel DI , Riessen R , Athanasiadis A , et al . Growth factor espression of human arterial smooth cells and endothelial cells in a transfilter coculture system[J ] . J Mol Cell Cardiol ,1997 ,29 :2967 - 2978.

[3 ] Gorog P , Kovacs IB. Inhibition of vascular smooth muscle cell migration by intact endothelium is nitric oxide-mediated : interference by oxidised low density lipoproteins[J ] . J Vasc Res , 1998 ,35 :165 - 169.

[4 ] Inoue K, Cynshi O , Kawabe Y, et al . Effect of BO-653 and probucol on c-MYC and PDGF-A messenger RNA of the iliac artery after balloon denudation in cholesterol-fed rabbits [J ] . Atherosclerosis , 2002 ,161 : 353 - 363.

[5 ] Ronnov-Jessen L , Petersen OW. Afunction for filamentous alpha-smooth muscle actin : retardation of motility in fibroblasts [ J ] . J Cell Biol ,1996 ,134 :67 - 80.

[6 ] Li Z , Cheng H , Lederer WJ , et al . Enhanced proliferation and migration and altered cytoskeletal proteins in early passage smooth muscle cells from young and old rat aortic explants[J ] . Exp Mol Pathol ,1997 ,64 :1- 11.

[7 ] Kingsley K, Huff JL , Rust WL , et al . ERK1/ 2 mediates PDGF-BB stimulated vascular smooth muscle cell proliferation and migration on laminin-5[J ] . Biochem Biophys Res Commun ,2002 ,293 :1000 - 1006.

[8 ] Berrou E , Bryckaert M. Platelet-derived growth factor inhibits smooth muscle cell adhesion to fibronectin by ERK-dependent and ERK-independent pathways[J ] . J Biol Chem ,2001 ,276 :39303 - 39309.

[9 ] Worth NF , Rolfe BE , Song J , et al . Vascular smooth muscle cell phenotypic modulation in culture is associated with reorganisation of contractile and cytoskeletal proteins[J ] . Cell Motil Cytoskeleton ,2001 ,49 :130- 145.

[10 ]王　睿,高　歌. 冠状动脉再狭窄的防治研究[J ] . 心脏杂志,2000 ,12 :221 - 223.

备注/Memo

备注/Memo:
收稿日期：2003-9-11
更新日期/Last Update: 2004-09-01