我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

不同试剂脱除猪胸主动脉壁细胞的对比研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2005年第3期
页码:
207-209
栏目:
基础研究
出版日期:
2005-05-05

文章信息/Info

Title:
Primary study on cell extraction from porcine thoracic aortas
作者:
顾春虎;  刘维永;  张近宝;  王云雅;  刘洋;  谭红梅;  宿学家
第四军医大学西京医院心脏外科, 陕西 西安 710032
Author(s):
GU Chunhu ; LIU Weiyong; ZHANG Jinbao; WANG Yunya; LIU Yang; TAN Hongmei; SU Xuejia
Department of Cardiology Surgery, Xijing Hospital, Fourth Military Medical University,Xi’an, Shaanxi 710032,China
关键词:
胰蛋白酶; 曲拉通X100; 组织工程; 血管; 脱细胞基质
Keywords:
trypsin; triton X100; tissue engineering; vessel; acellular tissue matrix
分类号:
R318.1
DOI:
-
文献标识码:
A
摘要:
目的 用不同方法去除猪胸主动脉壁细胞，并比较去细胞效果，为组织工程瓣膜或带瓣管道提供良好的实验材料。方法 采用十二烷基硫酸钠、胆脂酸钠、曲拉通（TritonX100）制备猪胸主动脉脱细胞基质。将实验分为十二烷基硫酸钠组、胆脂酸钠、曲拉通组和空白对照组。标本进行大体、光镜、电镜观察，力学性能的检测并做比较。结果 胆脂酸钠无法完全脱除主动脉壁内细胞，十二烷基硫酸钠和曲拉通都能完全脱除猪胸主动脉细胞，曲拉通较好地保持了胶原纤维和弹性纤维的原有排列和分布，并有较好的力学性能。后两者脱细胞时间无显著差别。结论 曲拉通可以成功用于制备大血管脱细胞基质，为组织工程瓣膜或带瓣管道的研究提供材料。
Abstract:
AIM To investigate methods to remove cellular compenents from porcine thoracic aortas,resulting scaffolds of acellular tissue matrix(ACTM) for tissue engneering of heart valve or vessel. METHODS Different detergents (Triton X100,Sodium dodecyl sulfate and sodium deoxycholate)and enzyme (trypsin) extraction was practiced in this study. According to the different detergents, specimens of porcine thoracic aortas devided into three groups(Sodium dodecyl sulfate,sodium deoxycholate,Triton X100). Specimens were observed grossly and the changes of mechanical properties were also studied.Haematoxylineosin a was performed to confirm the removal of cells. Transmission electron microscope was used to observe the integrity of collagen and elastin. RESULTS The cells were removed effectively from porcine thoracic aortas by Triton X100 and sodium dodecyl sulfate, and histological analysis showed that the major structural compenents of porcine thoracic aortas decelluarized by Triton X100 had been maintained.While sodium deoxycholate were failed for porcine thoracic aortas decellularization. the collagen and elastin were kept intact. CONCLUSION ACTM of vessel can be obtained successfully through this procedure by Triton X100.

参考文献/References

［1］Richard BC,David RA,Graham RN. Use of the medtronic freestyle valve as a right ventricular to pulmonary artery conduit［J］. Ann Thorac Surg， 2001，71:s361-s364.

［2］Kim WG, Park JK, Lee WY. Tissueengineered heart valve leaflets: an effective method of obtaining acellularized valve xenografts［J］. Int J Artif Organs， 2002，25(8):791-797.

［3］Korossis SA, Booth C,Wilcox HE,et al. Tissue engineering of cardiac valve prosthesesⅡ，b characterization of an acellular porcine iomechanical characterization of decellualrized porcine aortic heart valves［J］. J Heart Valve Dis,2002,11:463-471.

［4］Booth C,Korossis SA,Wilcox HE,et al. Tissue engineering of cardiac valve prostheses Ⅰ，development and histological characterization of an acellular porcine scaffold［J］. J Heart Valve Dis,2002,11:457-462.

［5］韩雪峰，杨大平，郭铁芳. 曲拉通X100对制备脱细胞血管基质影响的实验研究［J］. 中华外科杂志，2002，40：27-29.

［6］Reddy PP,Barrieras DJ,Wilson G,et al. Regeneration of functional bladder substitutes using large segment acellular matrix allografts in a porcine model［J］. J Urol,2000,164:936-941.

［7］Teebken OE, Bader A. Steinhoff G,et al. Tissue engineering of vascular grafts: human cell seeding of decellularised porcine matrix［J］. Eur J Vasc Endovasc Surg,2000,19: 381-386.

［8］Badylak SF,Park K, Peppas N, et al. Marrow-derived cells populate scaffolds composed of xenogeneic extracellular matrix［J］. Experimental Hematology, 2001,29:1310-1318.

备注/Memo

备注/Memo:
收稿日期：2004-07-06.基金项目：国家自然科学基金资助项目：（编号30271292）
更新日期/Last Update: 2010-01-05