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bFGF促进缺血心肌血管生成和改善心肌功能的实验研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2006年第3期
页码:
280-283
栏目:
基础研究
出版日期:
2006-06-25

文章信息/Info

Title:
Effect of basic fibroblast growth factor on angiogenesis in ischeamic myocardium and cardiac function
作者:
刘莹1; 2; 孙立军1; 宦怡1; 赵海涛1; 邓敬兰2
第四军医大学西京医院： 1. 放射科，2. 核医学科，陕西 西安 710032
Author(s):
LIU Ying1; 2;  SUN Li-jun1;  HUAN Yi1;  ZHAO Hai-tao1;  DENG Jing-lan2
1. Department of Radiology, 2. Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032， China
关键词:
心肌梗塞; 碱性成纤维生长因子; MR电影
Keywords:
myocardial infarction;  basic fibroblast growth factor;  cine magnetic resonance
分类号:
R542.22
DOI:
-
文献标识码:
A
摘要:
目的 利用生长因子通过诱导血管生成治疗缺血性心脏病，但生长因子的输入方式还存在一些争议。本研究旨在探讨心肌内注射碱性成纤维生长因子（bFGF）对急性心肌梗死(AMI)血管生成和心肌功能的作用。 方法 AMI犬24只，随机分成对照组（MI区注射生理盐水15 ml）和实验组（MI区注射50 mg bFGF与生理盐水的混合液15 ml）；每组观察4个不同的时间点（术后0、2、9、16 d）。各组动物分别在处死前应用敏感编码技术（sensitivity encoded technique，SENSE）行磁共振电影成像（cine magnetic resonance imaging，cineMRI）；电镜直接观察新生血管生长情况；免疫组织化学方法检测各组微血管数量；以左心室射血分数（LVEF）评价心脏功能的改变。 结果 实验组LVEF自9 d明显增加［9 d：对照组（24±3）%、实验组（46±6）%；16 d：对照组（31±5）%、实验组（53±5）%］；除0 d外各个时间点的微血管数量实验组均比对照组明显增多［2 d：对照组（92±12）支/视野、实验组（147±12）支/视野；9 d：对照组（125±12）支/视野、实验组（183±14）支/视野；16 d：对照组（125±14）支/视野、实验组（224±20）支/视野］。 结论 心肌内注射bFGF具有促进MI区域毛细血管形成及改善左心室功能的作用。
Abstract:
AIM Therapeutic angiogenesis with growth factors has described a strategy where blood vessel formation is induced for the purpose of treating ischemic heart diseases. However, the delivery strategies to achieve clinically significant angiogenic responses are controversial. The aim of the present study was to evaluate the effect of intramyocardial injection of basic fibroblast growth factor on angiogenesis in infarcted myocardium and cardiac function. METHODS Twentyfour mongrel dogs were randomized into control group and therapeutic group. Acute myocardial infarction was made by ligation of the left anterior descending coronary artery distal to its first diagonal branch. After coronary artery was occluded, 50 g of basic fibroblast growth factor (bFGF) in 15 ml of saline was injected into the infarcted myocardium and its periinfarct region in therapeutic group, whereas 15ml saline alone was used in control dogs. Every 3 dogs in each group were studied on 0, 2, 9 and 16 day after surgery respectively. With sensitivity encoded technique, cine MR was performed on each dog before euthanasia to evaluate cardiac function. Electron microscope was used to observe the growth of capillaries. Angiogenesis was evaluated by immunohistochemical studies with Ⅷ factor. RESULTS In therapeutic group, LVEF improved markedly 9 days after injection of bFGF. The capillaries grew actively in therapeutic group and microvessel density was higher in therapeutic group than in control group but the first day（2 day：control group 92±12, therapeutic group 147±12；9 day：control group 125±12, therapeutic group 183±14；16 day：control group 125±14, therapeutic group 224±20）. CONCLUSION Intramyocardial administration of bFGF is useful for increase in the growth of microvessels and improvement in left ventricular function in acute myocardial infarction.

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备注/Memo

备注/Memo:
收稿日期：2005-12-26.基金项目：陕西省自然科研基金项目资助(No.2003C2037) 通讯作者：孙立军，主任医师，主要从事心血管影像学研究 Tel: (029)84773592 Email: slj@fmmu.edu.cn 作者简介：刘莹，住院医师，博士 Tel: (029)84775414 Email: yingyinglyly@126.com
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