我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

罗格列酮对大鼠颈动脉球囊损伤后平滑肌细胞增殖和调亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2007年第5期
页码:
510-512/516
栏目:
基础研究
出版日期:
2007-10-01

文章信息/Info

Title:
Effect of Rosiglitazone on proliferation and apoptosis of vascular smooth muscle cells after carotid artery balloon injury in rats
作者:
布伦;  贾国良; 韩淑芳; 张薇; 王海昌
第四军医大学西京医院心内科，陕西 西安 710032
Author(s):
BU Lun;  JIA Guoliang;  HAN Shufang;  ZHANG Wei;  WANG Haichang
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, Shaanxi, China
关键词:
罗格列酮; 颈动脉损伤; 平滑肌细胞; 大鼠; 细胞增殖; 细胞凋亡
Keywords:
Rosiglitazone; carotid artery injury; smooth muscle cells;  rats; cell proliferation;  cell apoptosis
分类号:
R543.4
DOI:
-
文献标识码:
A
摘要:
目的 观察罗格列酮对大鼠颈动脉损伤后平滑肌细胞增殖和凋亡的影响。方法 SD大鼠随机分为3组，即对照组、手术组、治疗组。手术组及治疗组均给予左侧颈总动脉球囊损伤，治疗组给予罗格列酮灌胃治疗。术后2周取损伤血管行HE染色及光镜观察，计算内膜面积（NIA）、内弹力板围绕面积（IELA）及管腔狭窄指数（SI）。免疫组化法检测平滑肌细胞增殖率，Tunnel法检测平滑肌细胞凋亡率。结果 术后2周，手术组内膜面积、管腔狭窄指数显著高于对照组(P<0.01)；经治疗后，治疗组内膜面积、管腔狭窄指数显著低于手术组(P<0.01)。手术组细胞增殖率显著高于对照组(P<0.01)，而治疗组细胞增殖率显著低于手术组(P<0.01)。手术组细胞凋亡率显著低于对照组(P<0.01)，而治疗组细胞凋亡率显著高于手术组(P<0.01)。结论 罗格列酮可以促进血管平滑肌细胞凋亡，抑制其增殖，从而减轻损伤血管的再狭窄。
Abstract:
AIM To investigate the effect of Rosiglitazone on the proliferation and apoptosis of vascular smooth muscle cells after carotid artery injury in rats. METHODSS D rats were divided randomly into 3 groups: control group, surgery group and therapy group. The left common carotid arteries were injured by balloon in surgery group and therapy group and Rosiglitazone was administrated intragastricly to therapy group. After 2 weeks, the injured vessels were stained by HE staining method and observed with microscope. Neointimal area (NIA), internal elastic lamina area (IELA) and stenosis index (SI) were calculated. The proliferation rate of smooth muscle cells was examined with immunity histochemistry, and the apoptosis rate of smooth muscle cells was examined with Tunnel method. RESULTS After the 2week treatment, the NIA and SI of surgery group were significantly increased compared with those of control group (P<0.01). After the treatment, the NIA and SI of therapy group were significantly decreased compared with those of surgery group (P<0.01). The proliferation rate of surgery group was significantly increased compared with that of control group (P<0.01) and the proliferation rate of therapy group was significantly decreased compared with that of surgery group (P<0.01). The apoptosis rate of surgery group was significantly decreased compared with that of control group (P<0.01) and the apoptosis rate of therapy group was significantly increased compared with that of surgery group (P<0.01). CONCLUSION Rosiglitazone promotes the apoptosis of vascular smooth muscle cells and inhibits the proliferation of those cells, thus alleviating the restenosis of the injured vessels.

参考文献/References

［1］ Durand E, Mallat Z, Addad F, et al . Time courses of apoptosis and cell proliferation and their relationship to arterial remodeling and restenosis after angioplasty in an atherosclerotic rabbit model［J］. J Am Coll Cardiol, 2002, 39(10):1680-1685.

［2］ Lindner V, Fingerle J, Reidy MA. Mouse model of arterial injury［J］. Circ Res, 1993, 73(5):792-796.

［3］ Law RE, Meehan WP, Xi XP, et al .Troglitazone inhibits vascular smooth muscle cell growth and intimal hyperplasia［J］. J Clin Invest, 1996, 98(8):1897-1905.

［4］ Goetze S, Xi XP, Kawano H, et al . PPAR gammaligands inhibit migration mediated by multiple chemoattractants in vascular smooth muscle cells［J］. J Cardiovasc Pharm, 1999, 33(5):798-806.

［5］ MartinNizard F, Furman C, Delerive P, et al . Peroxisome proliferatoractivated receptor activators inhibit oxidized lowdensity lipoproteininduced endothelin1 secretion in endothelial cells［J］. J Cardiovasc Pharmacol, 2002, 40(6):822-831.

［6］ Aizawa Y, Kawabe J, Hasebe N, et al . Pioglitazone enhances cytokineinduced apoptosis in vascular smooth muscle cells and reduces intimal hyperplasia［J］. Circulation, 2001, 104(4):455-560.

［7］ Redondo S, Ruiz E, SantosGallego CG, et al . Pioglitazone induces vascular smooth muscle cell apoptosis through a peroxisome proliferatoractivated receptorgamma, transforming growth factorbeta1, and a Smad2dependent mechanism［J］. Diabetes, 2005, 54(3):811-817.

［8］ Takagi T, Yamamuro A, Tamita K, et al. Thiazolidinedione treatment attenuates diffuse neointimal hyperplasia in restenotic lesions after coronary stent implantation in type 2 diabetic patients: an intravascular ultrasound study［J］. J Cardiol, 2005, 45(4):139-147.

备注/Memo

备注/Memo:
收稿日期：2006-05-11.通讯作者： 贾国良，主任医师，主要从事冠脉狭窄研究Email: jiagl@126.com 作者简介：布伦，博士，主治医师Email: bulun75@126.com
更新日期/Last Update: