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Title:: Effect of Ginsenoside Rg1 in promoting myocardiac regeneration in myocardial infarction rats

作者:: 杨敏; 陈广玲; 陈畅; 张钰; 闫纯英; 汕头大学医学院第一附属医院心血管内科，广东汕头 515041

Author(s):: YANG Min; CHEN GuangLing; CHEN Chang; ZHANG Yu; YAN ChunYing; Department of Cardiology, First Affiliated Hospital, Medical College, Shantou University, Shantou 515041, Guangdong, China

关键词:: 心肌梗死; 人参皂甙Rg1; 干细胞; 心肌再生; 心室重构

Keywords:: myocardial infarction; ginsenoside Rg; stem cell; regeneration of myocardium; ventricular remodeling

分类号:: R542.22

DOI:: -

文献标识码:: A

摘要:: 目的观察人参皂甙Rg1促进大鼠心肌梗死后缺血心肌的再生。方法结扎Wistar大鼠左冠状动脉制作急性心肌梗死（acute myocardial infarction，AMI）模型，随机分为治疗组和对照组，治疗组大鼠采用人参皂甙Rg1治疗。于建模后24 h、2周和4周时杀死大鼠，取出心脏，HE染色观察细胞形态特征和心肌基本结构，并测量梗死面积，计算心室肌质量/体质量的比值、心肌梗死总面积/左心室肌总面积的百分比；免疫组织化学方法观察心肌组织CD34阳性细胞的表达；并比较治疗前后外周血中CD34阳性细胞数量的变化。结果应用人参皂甙Rg1治疗后，外周血中CD34细胞数量变化：治疗组，制模前: （0.043±0.023）％，24 h: （0.202±0.081）％，2周: （0.937±0.142）％，4周: （0.834±0.110）％；对照组，制模前: （0.046±0.022）％，24 h: （0.056±0.037）％，2周: （0.069±0.045）％，4周: （0.064±0.042）％；治疗组制模后24 h、2周和4周的CD34阳性细胞百分率均比制模前和对照组大鼠的百分率明显升高（P<0.05）；治疗组大鼠在第2、4周时，心室肌质量/体质量均比对照组明显低（P<0.05）；治疗组大鼠心梗区可见大量CD34阳性细胞浸润，其梗死面积明显减小；治疗组大鼠心肌梗死程度较对照组减轻，其缺血心肌的基本结构得到保护。结论应用人参皂甙Rg1治疗AMI大鼠，能显著提高外周血的干细胞数量，并促进干细胞归巢梗死心肌、分化为心肌细胞样细胞，促进心肌再生缩小梗死面积，明显减轻心室重构，保护缺血心肌的基本结构。

Abstract:: AIM To investigate the treatment effects of ginsenoside Rg1 on rat ischemic myocardium after acute myocardial infarction (AMI). METHODS Left anterior descending coronary arteries were ligated to produce AMI model in wistar rats. The rats were randomly divided into 2 groups: the treatment group and the control group. Bone marrow stem cells were mobilized by ginsenoside Rg1and stem cells homed to the site of myocardial infarction. Hearts were harvested in the 24th hour, 2nd and 4th week after AMI modeling for histopathological examination. Immunohistochemisty and HE stain were used to detect infiltration of CD34＋cells (the surface antigenic markers of stem cell) and the regeneration of myocytes in the part of ischemia. Comparison between the two groups was made in terms of ratio of ventricular weight to body weight, and percentage of the infarction area to the total surface area of the LV. CD34+ cell numbers in peripheral blood before and after modeling was determined with flowcytometry. RESULTS The percentage of CD34＋cells in peripheral blood after 24th hour and 4th week of preparation of rat myocardial infarction model: \[the treatment group, before treatment: (0.043±0.023)％, 24 h: (0.202±0.081)％, 2 weeks: (0.937±0.142)％, 4 weeks: (0.834±0.110)％; the control group, before modeling: (0.046±0.022)％, 24 h: (0.056±0.037)％, 2 weeks: (0.069±0.045)％, 4 weeks: (0.064±0.042)％\]. It was significant higher (P<0.05）than that before treatment and in AMI group. Infarct area in treatment group was obviously smaller than that in AMI group. There were a great number of monocytes infiltrating with CD34 expression by immunohistochemisty in myocardial infracted zone in the treated rats. In the 2 nd and 4 th week, the ratio of ventricular weight to body weight in the treatment group was smaller than that in the control（P<0.05）. The necrosis in treatment group was significantly slighter than that in control group and only limited to subendothelium. CONCLUSION After treatment with ginsenoside Rg1, the number of CD34＋cells increases significantly in peripheral blood and the ability of bone marrow stem cells homing to infracted myocardium increases. Ginsenoside Rg1 mobilizes many bone marrow stem cells to home to infracted zones and differentiate to cardiomyocytelike cells. Ischemic myocardial structure is protected and ventricular remodeling is attenuated.

参考文献/References

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人参皂甙Rg1可促进大鼠心肌梗死后心肌再生(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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