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参松养心胶囊对Kv1.4钾电流特性的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2009年第6期
页码:
782-785,789
栏目:
基础研究
出版日期:
2009-11-05

文章信息/Info

Title:
Effects of shensongyangxin capsule on c-type Kv1.4 potassium channel
作者:
王智泉1; 蒋学俊2; 巢升平1; 任江华1; 曹茂银1; 张冬1
武汉大学：1.中南医院心内科，湖北 武汉 430071，2.人民医院心内科，湖北 武汉 430060
Author(s):
WANG Zhi-quan1;  JIANG Xue-jun2;  CHAO Sheng-ping1;  REN Jiang-hua1;  CAO Mao-yin1;  ZHANG Dong1
1.Department of Cardiology, Zhongnan Hospital, Wuhan 430071, Hubei, China, 2.Department of Cardiology, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei, China
关键词:
参松养心胶囊; 钾通道; 电压门控型离子通道; 电压钳; 膜电流
Keywords:
shensongyangxin;  potassium channel;  voltage-gated ion channels;  voltage clamp;  membrane currents
分类号:
R541.7
DOI:
-
文献标识码:
A
摘要:
目的: 探讨中药复方制剂参松养心胶囊对KV1.4钾通道C型(KV1.4△N)失活的影响。方法: 将Kv1.4ΔN mRNA注射入非洲爪蟾卵母细胞中，使用双电极钳制法（Two electrodes voltage clamp TEV）观察参松养心胶囊对KV1.4ΔN电生理特性的影响 。结果: 参松养心胶囊对Kv1.4ΔN通道的峰电流有抑制作用，这种阻滞作用具有电压依赖性,随电位的升高而作用加强，符合单指数和线性关系。参松养心胶囊可加速KV1.4ΔN钾通道电流的失活过程,同时可使KV1.4ΔN通道失活后的恢复减慢 。结论: 参松养心胶囊能显著抑制KV1.4钾通道电流，这可能是其抗心律失常作用的机制之一。
Abstract:
AIM: To study the effects of shensongyangxin capsule, a traditional Chinese medicine, on c-type Kv1.4 channels in xenopus laevis oocytes by two-electrode voltage-clamp technique. METHODS: Defolliculated oocytes (stages V VI) were injected with transcribed cRNAs of ferret Kv1.4△N channels. During recording, oocytes were continuously perfused with control solution or shensongyangxin. RESULTS: Shensongyangxin decreased the currents during voltage steps in a voltage-dependent manner. The block increased with positive potentials and the voltage dependence of block matched the sum of monoexponentials and linear relationships. Shensongyangxin accelerated the inactivation of current during the voltage step and prolonged the time of recovery from the inactivation. CONCLUSION: These results suggest that shensongyangxin blocks the Kv1.4△N channel, which may underly its antiarrhythmic mechanisms.

参考文献/References

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［2］ 吴以岭，谷春华，高学东,等. 参松养心胶囊治疗冠心病室性早搏的随机双盲多中心临床研究［J］. 疑难病杂志, 2007, 6(8):449-452.

［3］ 李宁,吴相锋,马克娟,等. 参松养心胶囊对心室肌细胞钾通道的影响［J］. 疑难病杂志， 2007， 6（3）：133-137.

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［5］ Rasmusson RL, Morales MJ, Castellino RC, et al. C-type inactivation controls recovery in a fast inactivation cardic K+ channel (Kv1.4) expressed in Xenopus oocytes［J］. J physiol, 1995, 489(Pt 3):709-721.

［6］ Labro AJ, Grottesi A, Sansom MS, et al. A Kv channel with an altered activation gate sequence displays both “fast” and “slow” activation kinetics［J］. Am J Physiol Cell Physiol, 2008, 294(6):C1476-C1484.

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［9］ Herrera D, Mamarbachi A, Simoes M, et al. A single residue in the S6 transmembrane domain governs the differential flecainide sensitivity of voltage-gated potassium channels［J］. Mol Pharmacol, 2005, 68(2):305-316.

备注/Memo

备注/Memo:
收稿日期：2009-3-4.基金项目：国家自然科学基金项目资助(30270362) 作者简介：王智泉，博士Email:wangzhiquan1031@yahoo.com.cn
更新日期/Last Update: 2009-09-30