生长分化因子11在白色脂肪细胞棕色化中的作用研究
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2015年第5期
- 页码:
- 524-527
- 栏目:
- 基础研究
- 出版日期:
- 2015-05-05
文章信息/Info
- Title:
- Role of GDF11 in transformation of white adipocytes to brown adipocytes
- Author(s):
- YAN Bing-wen; LIU Yi; PEI Hai-feng; WANG Shan; ZHANG Ling; TAO Ling
- (Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
- Keywords:
- GDF11; white adipocytes; brown adipocytes; transformation
- 分类号:
- R363
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 探讨生长分化因子11（GDF11）在白色脂肪细胞棕色化中的作用。方法 由C3H10T1/2细胞诱导分化而来的成熟白色脂肪细胞随机分为正常对照组，GDF11组；Western blot检测GDF11孵育成熟白色脂肪细胞后UCP1蛋白的表达；实时PCR检测GDF11对棕色脂肪标志基因表达的影响。结果 分别加入浓度为25 ng/ml、50 ng/ml、100 ng/ml 的GDF11，与正常对照组相比加入100 ng/ml的GDF11能够明显促进UCP1的表达（P<0.05），而25 ng/ml和50 ng/ml的GDF11可以在一定程度上诱导UCP1的表达却无显著统计学差异；同时，100 ng/ml的GDF11能够促进棕色脂肪标志基因ucp1、cidea、cox7α、dio2和elovl3的表达（P<0.05）和线粒体DNA拷贝数的增加（P<0.05）。结论 GDF11可显著促进白色脂肪细胞向棕色脂肪细胞转化。
- Abstract:
- AIM To investigate the role of growth differentiation factor 11 (GDF11) in transformation of white adipocytes to brown adipocytes. METHODS Mature white adipocytes were obtained from differentiated C3H10T1/2 and were randomly divided into normal control group and GDF11 group. The expression of UCP1 in mature white adipocytes incubated with GDF11 was detected by Western blot and the expression of brown adipose gene was measured by real-time PCR. RESULTS GDF11 was added at 25, 50, and 100 ng/ml, respectively. Compared with control group, expression of UCP1 increased significantly at 100 ng/ml in GDF11 group (P<0.05), whereas there was no significant difference at 25 ng/ml and 50 ng/ml. GDF11 promoted the expression of brown adipose markers ucp1, cidea, cox7α, dio2 and elovl3 at 100 ng/ml (P<0.05). CONCLUSION GDF11 significantly promotes the transformation of white adipocytes into brown adipocytes.
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备注/Memo
- 备注/Memo:
- 收稿日期：2015-01-26.
基金资助：国家杰出青年科学基金项目资助(81225001)；国家自然科学基金项目资助(81170186)
通讯作者：陶凌，主任医师，主要从事糖尿病与心血管保护研究 Email: lingtao2006@ gmail.com
作者简介：闫柄文，硕士生 Email：380461196@qq.com
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