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miR-148b参与心肌梗死后心脏纤维化的作用及机制(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第2期
页码:
149-153
栏目:
基础研究
出版日期:
2016-11-25

文章信息/Info

Title:
Role and mechanism of miR-148b involved in cardiac fibrosis after myocardial infarction
作者:
赵 娜1; 李 娜2; 刘小军1; 徐姣姣1; 夏东雨1; 牛小麟3; 刘仲伟1
（1.陕西省人民医院心内一科，陕西 西安 710068；
西安交通大学：2.第一附属医院心内科，陕西 西安 710061，3.第二附属医院心内科，陕西 西安 710004）
Author(s):
ZHAO Na1;  LI Na2;  LIU Xiao-jun1;  XU Jiao-jiao1;  XIA Dong-yu1;  NIU Xiao-lin3;  LIU Zhong-wei1
(1.Department of Cardiology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi, China；
2.Department of Cardiology, First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China；
3.Department of Cardiology, Second Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Xi’an 710004, Shaanxi, China)
关键词:
心肌梗死; 心脏纤维化; miR-148b; PTEN
Keywords:
myocardial infarction;  cardiac fibrosis;  miR-148b;  PTEN
分类号:
R734.2
DOI:
-
文献标识码:
A
摘要:
目的 探讨miR-148b参与大鼠心肌梗死后心脏纤维化的作用及可能机制。方法 采用结扎大鼠冠状动脉左前降支方法制作大鼠心肌梗死模型，RT-PCR法评价大鼠心脏miR-148b表达水平；应用生物信息学方法预测miR-148b靶基因；采用Western blot方法评价大鼠心脏同源性磷酸酶-张力蛋白（phosphatase and tensin homologue，PTEN）及α-平滑肌蛋白（α-smooth muscle actin，α-SMA）蛋白表达水平；四甲基偶氮唑盐比色（MTT）法检测心脏成纤维细胞增殖能力；应用天狼猩红染色评价心脏纤维化病理改变。结果 大鼠心肌梗死组，梗死周边区纤维化程度显著增加，miR-148b表达显著上调（P<0.01）；miR-148b与预测靶基因PTEN mRNA有结合位点；大鼠心肌梗死组，梗死周边区PTEN表达显著下调（P<0.01）；在心脏成纤维细胞中过表达miR-148b，PTEN的蛋白表达水平显著下调（P<0.01），α-SMA蛋白表达水平显著上调（P<0.01），细胞增殖能力显著增强（P<0.05）；在AngII诱导心肌纤维化细胞模型中，抑制miR-148b,PTEN蛋白表达水平显著上调（P<0.05），α-SMA蛋白表达水平显著下调（P<0.01），心脏成纤维细胞增殖显著减少（P<0.01）。结论 miR-148b通过PTEN参与心肌梗死后心脏纤维化作用。
Abstract:
AIM To explore the role and mechanisms of miR-148b involved in cardiac fibrosis after myocardial infarction. METHODS The myocardial infarction model was induced by ligation of the left anterior descending coronary artery of rats. The target of miR-148b was predicted using bioinformatic methods. The expression level of miR-148b in rat heart tissue was evaluated using real-time PCR. The expression level of PTEN and α-SMA protein in rat heart tissue was evaluated using Western blot. Cardiac fibroblast proliferative ability was assessed using MTT assay and cardiac fibrosis was analyzed using Sirius red staining. RESULTS After myocardial infarction, fibrosis tissue increased and expression of miR-148b was up-regulated significantly in the border zone (P<0.01). The binding sites between miR-148b and PTEN mRNA were found. After myocardial infarction, the expression of PTEN was significantly down-regulated in the border zone (P<0.01). In the cardiac fibroblasts, miR-148b was overexpressed, PTEN protein expression level was significantly down-regulated (P<0.01), α-SMA protein expression level was significantly up-regulated (P<0.01) and the proliferation of cardiac fibroblasts significantly increased (P<0.05). In the cardiac fibroblasts stimulated by AngII, miR-148b was inhibited, PTEN protein expression level was significantly up-regulated (P<0.05), α-SMA protein expression level was significantly down-regulated (P<0.01) and the proliferation of cardiac fibroblasts was significantly decreased (P<0.01). CONCLUSION miR-148b is involved in cardiac fibrosis via PTEN after myocardial infarction.

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备注/Memo

备注/Memo:
收稿日期：2015-11-23.
基金项目：国家自然科学青年基金项目资助（81400181）
通讯作者：刘仲伟，主治医师，主要从事心力衰竭及糖尿病心肌病研究 Email:liuzhongweicn@gmail.com
作者简介：赵娜，主治医师，博士 Email：173307845@qq.com
更新日期/Last Update: 2016-10-11