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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2001年第4期

页码:

315-316，325

栏目:

博士之窗

出版日期:

2001-07-01

文章信息/Info

Title:

Effects of CGRP on cardiac Ca2+ channel on normal，imitating ischemia and hypoxia conditions and simulation on mathematical model of cardiac myocytes

作者:

商立军

第四军医大学基础部生理学教研室，陕西西安710032

Author(s):

SHANG Li-jun; ZANG Yi-min; DONG Xiu-zhen

Department of Physiology，Fourth Military Medical University，Xi'an Shaanxi 710032，China

关键词:

降钙素基因相关肚; 缺血; 缺氧; 急性分离; 心肌细胞; 钙通道; 离子电流; 数学模型

Keywords:

caleitonin gene-related peptide(CGRP); isehemia; hypoxia; acute isolation; cardiomyocytes; calcium ehanne1; ionic current; mathematiea1 model

分类号:

R331.36；TP391.9

DOI:

-

文献标识码:

A

摘要:

作者运用细胞培养、膜片钳和激光共聚焦技术，观察了降钙索基因相关肽(CGRP)对正常及模拟缺血、缺氧心肌细胞钙通道的作用及其电生理离子机制，并利用计算机重建技术在心肌细胞通道动力学及电流重建等方面进行了探索性研究．得到了如下提示性结论：①CGRP对心肌的保护作用有赖于适当剂量和浓度；② 本课题建立的急性心肌细胞分离方法，能够得到具有正常电生理特性的耐钙心肌细胞，是膜片钳实验和激光扫描共聚焦技术测定胞内钙的基础；③CGRP对急性缺血、缺氧心肌细胞具有保护作用，在急性缺血、缺氧时，急性分离的心肌细胞胞内钙离子浓度降低，CGRP可引起胞内钙浓度稍微升高，且CGRP可改善因急性缺血、缺氧引起的胞内钙浓度的降低，其机制有待于进一步探讨；①CGRP能促使正常及模拟缺血缺氧状态下豚鼠心室肌细胞ICa内流的增加,是CGRP正性作用的主要离子机制；CGRP对钙通道的作用机制还有待进一步研究；②本课题建立的对几种离子电流的计算机重建方法，提示可以被用来讨论离子电流机制，是一种新的途径。

Abstract:

By using cell culture，the patch clamp technique and laser scanning confocal technique,this study focused on the effects of calcitonin gene-related peptide(CGRP)on cardiac calcium channel on normal condition as well as on imitating ischemia and hypoxia conditions and the electrophysiologlcal ionic mechanisms． Meanwhile by using computer construction technique，this study tried to reconstruct channel dynamic courses and currents．The studies suggested that：①The appropriate dosage and concentration of CGRP showed protective effect on culture cardiomyocytes；②The isolation method established in this study can provide calcium-tolerant single ventricular myocytes which had normal electrophysiological properties and guaranteed a smooth operation of patch clamp and laser scanning confocal experiments；③CGRP had protective effect on acute hypoxia and ischemia cardiomyocytes．But these effects might be the jolnt result of many factors and need to be further investigated；④CGRP had significant enhancement effects on ICa-L in guinea-pig ventrieular myocytes under normal condition as well as under imitating ischemia and hypoxia conditions, which suggested that its effect might be the main ionic channel mechanism of its positive effects；⑤The mathematical models of all ionic currents established in this study could simulate the properties of ionic currents and channel dynamic courses properly and couldprovide some theoretica1 proofs for further anima1 experiments．

参考文献/References

[1]商立军．臧益民．降钙素基因相关肽对培养心肌细胞作用的实验观察[J]. 第四军医大学学报，2001，20(3)：234．

[2]Lopez-lopez JR，Shacklock PS, Balke CW，et al.Local calcium transients trigered by single L-type calcium channel currents in cardiac cells[J]．Science，1995，268:l042-l045．

[3]刘华军，杨学义，陈晏珠，等。低氧复氧对豚鼠心肌细胞钾通道电流及钙通道电流的影响[J]。中华心血管病杂志．1997，25：85．

[4]Ohmura T，Nishin M，Kigoshi S，et a1．Electrophysiological and medical efects of calcitonin gene related peptide on guinea-pig atria[J]. Br J Pharmacol,1990,1OO：17.

[5]Droogmans G,Nilius B.Kinetic propertie of the cardiac T-type calcium channel in the guinea-pig[J]．J Physiol(lond)．1989，419，L627-650.

[6]商立军，臧益民,王四旺，等．各部位心肌细胞的分离技术[J]．心功能杂志．1990,11(3):182.

[7]商立军，臧益民，王四旺，等.降钙素基因相关肽与心律失常[J]．心功能杂志．1999．11(4)：250.

[8]商立军，臧益民，臧伟进，等.成熟心肌细胞培养的历史回顾及进展[J]．心脏杂志，2000，l2(11)：37．

[9]商立军，臧益民，朱妙章，等.培养的成熟心肌细胞的形志学与电生理特性[J]．心脏杂志，2000，12(2)：116．

[10]商立军，臧益民，王四旺．耐钙心肌细胞的分离及基本电生理特性[J]．第四军医大学学报．2000，21(2）：247．

[11]商立军，臧益民，王春梅，等.降钙素基因相关肽对心肌细胞缺血缺氧状态下胞内钙的影响[J].第四军医大学学报．2OO0，21(4):408．

[12]商立军,臧益民，臧伟进.CGRP对正常及模拟缺血缺氧状态下豚鼠心肌细胞钙通道电流的影响[J]．中国药理学通报2000，16(5）：503。

[13]商立军，臧益民，臧伟进，等．心肌延迟整流钾流Ik动力学机制的计算机重建[J]．心脏杂志，2000，l2(4)：255.

备注/Memo

备注/Memo:

收稿日期：2000-10-24.基金项目：国家自然科学基金(No.3977028l，No．39870334，No.39970273)

常用功能

更新日期/Last Update: 2001-07-01