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阿片受体κ亚型在大鼠心肌细胞热应激反应中的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第5期
页码:
357-359
栏目:
论著
出版日期:
2001-09-01

文章信息/Info

Title:
The effect of κ-opioid receptor in the heat stress in rat ventricular myocytes
作者:
周京军;  朱运龙;  裴建明;  高　瞻;  王跃民
第四军医大学生理学教研室, 陕西西安710032
Author(s):
ZHOU Jing-jun;  ZHU Yun-long ;  PEI Jian-ming ;  GAO Zhan; WANG Yue-min
Department of Physiology, the Fourth Military Medical University, Xi’an Shaanxi 710032,China
关键词:
U50488H;  心肌细胞;  热休克蛋白;  热应激
Keywords:
U50488H;  myocyte;  heat shock proteins;  heat stress
分类号:
Q25;Q274
DOI:
-
文献标识码:
A
摘要:
目的 U50488H 是阿片肽受体κ亚型的选择性激动剂, 本实验研究U50488H 预处理是否可增强心肌细胞对热应激的耐受性, 同时观察与热休克蛋白70 及90 表达的关系。方法 采用分离的大鼠心肌细胞作为研究对象,以心肌细胞释放乳酸脱氢酶(LDH) 的多少反映其损伤程度并用Westernblot 分析热休克蛋白表达的变化。结果 在5～ 30 Lmol/L 浓度范围内,U50488H 预处理30min 剂量依赖性的减低心肌细胞热应激后LDH 的释放。同时热休克蛋白70 和90 的表达明显增加。结论 U50488H 激活阿片受体后具有保护心肌细胞的作用, 其保护机制可能与调节热休克蛋白的表达有关。
Abstract:
AIM U50488H is the selective agonist of kappa opioid receptor. The present study was to investigate whether U50488H pretreatment increases rat ventricular myocytes to lerance against heat stress. METHODS Ventricular myocytes were isolated and pretreated with U50488H for 30 min, then they were subjected to heat stress at 44℃ for 30 min followed by 2 h culture at normal conditions (37℃). RESULTS The lactate dehydrogenase (LDH) release in the U50488H pretreatment group was decreased in the dose-dependent manner, and the expression of heat shock protein 70 and 90 were significantly increased. CONCLUSION Kappa opioid receptor activation with U50488H increases rat ventricular myocytes to lerance against heat stress with elevation of levels of HSP70 and HSP90.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-08-25.
更新日期/Last Update: 2001-09-01