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在人小肠上皮细胞膜上检出钙激活性钾通道(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第1期
页码:
7-12
栏目:
论著
出版日期:
2002-01-01

文章信息/Info

Title:
Identfication of a Ca2+-activated K+ channel in the cell membrane of human intestinal epithelial cells
作者:
王　军1; 徐洪涛2; 臧益民1
1. 第四军医大学生理学教研室, 陕西 西安 710032; 2. 海军总院心血管内科
Author(s):
WANG Jun1;  XU Hong-tao2;  ZANG Yi-min1
1. Department of Physiology, Fourth Military Medical University, Xi′an Shaanxi 710032, China;2. Department of Cardiovascular Disease,Beijing Navy Hospital
关键词:
人类小肠上皮细胞;  逆转录聚合酶链反应;  全细胞记录;  单通道记录
Keywords:
Human epithelial intestine 407 cells;  RT-PCR;  whole-cell recording;  single-channel recording
分类号:
-
DOI:
-
文献标识码:
A
摘要:
目的 研究人类小肠上皮细胞( Intestine 407 cell lines) 膜上钙激活性钾通道的分子生物学特征及其电生理学特性。方法 用RT 2PCR 方法检测培养的人类小肠上皮细胞膜上钙激活性钾通道的表达形式, 用膜片钳的全细胞记录及单通道记录法探讨了该通道的电生理学特点。结果 RT 2PCR 证实该细胞系有intermediate2conductance ( IK)钙激活性钾通道表达, 而没有large2conductance (BK) 和small2conductance (SK) 钙激活性钾通道的表达。电生理学研究表明, Ionomycin 引导的全细胞电流显示该钙激活性钾电流具有内向整流性并可被特异度IK 钙激活性钾通道阻断剂clo t rimazo le 所抑制, 但特异度SK 钙激活性钾通道阻断剂apam in 对该电流无明显抑制作用。单通道记录法证实该通道的电导为30±2 pS, 通道活性对细胞内Ca2+ 浓度有明显依赖性。结论 分子生物学和电生理研究证实了人类小肠上皮细胞膜上有IK 钙激活性钾通道, 其生理学意义有待进一步研究。
Abstract:
The physilogical significance of the Ca2+-activated K+ channels has been described in a various tissues especially in epithelial cells. Due to their sensitivity to Ca2+ , these channels are very important elements in the coupling of in tracellular Ca2+ signaling to the electrical properties of the cells. This study was aimed at identifying the molecular identity for the K+ channels in Intestine 407 cells. RT-PCR demonstrated that expression of an intermediate-conductance Ca2+-activated K+ channels, whole-cell recordings demonstrated a inward rectify K+ channel. Single channel data shown that its single channel conductance was 30±2 pS and its activity properties are consistent with intermediate-conductance Ca2+-activated K+ channel in human erythrocytes.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2001-08-28.
更新日期/Last Update: 2002-01-01