我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

新生动物同种主动脉带瓣管道移植的免疫反应与钙含量的相关性(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第1期
页码:
22-24
栏目:
论著
出版日期:
2002-01-01

文章信息/Info

Title:
The correlation between immune response and calium content in implanted valved aortic homograft conduit of neonte rabbit
作者:
陈文生;  刘维永;  汪　钢;  胡　军;  朱海龙;  谭红梅
第四军医大学西京医院心血管外科, 陕西 西安 710032
Author(s):
CHEN Wen-sheng ;  LIU Wei-yong ;  WANG Gang ;  HU Jun;  ZHU Hai-long ;  TAN Hong-mei
Department of Cardiovasclar Surgery, Xijing Hospital, Fourth Military Medical University,Xi’an Shaanxi 710032, China
关键词:
同种主动脉带瓣管道;  移植;  免疫反应;  钙化;  新生动物
Keywords:
valved aortic homograft conduit;  transplantation;  immunity;  calcification;  neonate animal
分类号:
R654.2
DOI:
-
文献标识码:
A
摘要:
目的 探讨新生动物同种主动脉带瓣管道(VAHC) 移植后免疫反应与钙含量之间关系。方法 体重150～ 200g 的新生新西兰兔24 只作为VAHC 的供体, 0. 9～ 1. 2 及2. 1～ 2. 9 kg 的幼兔和成年兔各12 只为受体, 建立新生兔的VAHC 移植模型。术后1 个月和6 个月分别取材作免疫荧光染色及钙含量测定。结果 免疫荧光检查显示各移植组VAHC 血管壁IgG 荧光染色结果均为阳性。半定量分析显示植入幼兔体内1 个月及成年兔1 个月和6 个月体内的阳性积分显著高于植入幼兔体内6 个月的VAHC (P < 0. 05)。各移植组VAHC 血管壁的钙含量及植入幼兔体内6 个月和成年兔体内瓣膜的钙含量显著高于对照组(P < 0. 05)。血管壁免疫荧光强度积分结果与钙含量呈明显的正相关( r= 0. 62, P < 0. 05)。结论 新生兔的VAHC 植入幼兔和成年兔体内后移植物的免疫反应程度与钙含量有明显的相关性。
Abstract:
AIM To investigate the crrelaion between immune respose and calcium content in implanted valved aortic homograft conduit (VAHC) of neonate rabbit. METHODS The animal model of VAHC was established via retroperitoneal pathway. Neonatal New Zealand rabbis(weight: 150～ 200 g, n= 24) were used as donors of the VAHCs, and young (0. 9～ 1. 2 kg, n=12) and adult rabbits (2. 1～ 2. 9 kg, n = 12) as recipients. The specimens were studied with immunofluorescence technique and their calcium contents were measured 1 month and 6 months after the implantation. RESULTS Immunofluorescence revealed the positive IgG fluorescent stain in VAHC walls of all the grafted groups. Semi-quantitative analysis showed that fluorescence stain in the VAHC walls of young recipients in 1 month and adult recipients after implantation was more intense than that in the VAHC walls of young recipients in 6 months after the implantation (P < 0. 05). The calcium content of VAHC walls in young recipients in 6 months and adult recipients was higher than that in the control group (P < 0. 05). The intensity of immunofluorescent stain and calcium content in the VAHC walls of all the grafted groups correlated obviously (R= 0. 62, P< 0. 05). CONCLUSION The intensity of immune response correlated well with the calcium content on implanted valved aortic homograft conduit (VAHC) of neonate rabbit.

参考文献/References

[1] 陈文生, 刘维永, 汪　钢, 等. 新生兔的同种主动脉带瓣管道移植模型[J]. 中华实验外科杂志, 1999, 16[增刊]: 88- 89.

[2] Lemstrom KB. Bruning. Triple drug immmunosuppression significantly reduces immune activation and allograft arteriosclerosis in cytomegalovirus-infected rat aortic allografts and induces early latency of viral infection [J]. Am J Pathology , 1994, 114 (8) : 1334- 1347.

[3] 易定华, 高双斌, 刘维永. 原子吸收火焰法测定异种生物瓣钙含量的方法[J]. 中华物理医学杂志, 1988, 10 (2) : 170- 172.

[4] Zhao XM , Green M. F razer IH, et al. Donor-specific immune response after aortic valve allografting in the rat [J]. Ann Thorac Surg , 1994, 57 (7) : 1158- 1163.

[5] Hamer ID, Hepkema B, Prop J , et al. HLA antibodies specific for cryopreserved heart valve“homografts”in children [J]. J Thorac Cardiovasc Surg , 1997; 113 (3) : 417- 419.

[6] Yankah AC, Wottge HU , Muler-ruchholtz W , et al. Short-course cyclosporin A therapy for definite allograft valve survival immunosuppression in allograft valves operations [J]. Ann Thorac Surg , 1995, 60 (Suppl) : 146- 150.

[7]Lupinetti FM , Cobb S. Effect of immunological differences on rat aortic valve allograft calcification [J]. J Cardial Surg , 1992,7 (1) : 65- 70.

[8] Clarke DR, Campbell DN ,Hayward AR, et al. Degeneration of aortic valve allografts in young recipients [J]. J Thorac Cardiovasc Surg , 1993, 105 (4) : 934- 942.

备注/Memo

备注/Memo:
收稿日期：2001-06-28.
更新日期/Last Update: 2002-01-01