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脂多糖对肺动脉内皮细胞Ⅰ型Na+/H+交换器表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第3期
页码:
199-201,204
栏目:
实验研究
出版日期:
2003-05-01

文章信息/Info

Title:
The changes of NHE-1 by lipopolysaccharide on pulmonary artery endothelial cells
作者:
赵澎涛; 李志超*; 刘毅; 李剑
第四军医大学基础部病理生理学教研室,陕西 西安 710032
Author(s):
Zhao Peng-tao; Li Zhi-chao; Liu-Yi; LiJian
Department of Pathophysiology,Faculty of Preclinical Medicine,Fourth Military Medical University ,Xi'an,Shaanxi 710032,China
关键词:
脂多糖; Ⅰ型Na+/H+交换器; 肺动脉内皮细胞; 钙离子
Keywords:
lipopolysaccharide;  sodium-hydrogen exchanger inform-1; pulmonary artery endotheliacells; calcium
分类号:
R322.12
DOI:
-
文献标识码:
A
摘要:
目的:探讨脂多糖(LPS)对培养的牛肺动脉内皮细胞(PAEC)Ⅰ型Na+/H+交换器(NHE-1)表达的影响及其与内皮细胞死亡的关系。方法:体外培养牛PAEC,MTT比色检测细胞存活率,并提取总RNA,应用反转录聚合酶链式反应(RT-PCR)及DNA电泳技术,以NHE-1与β-actin电泳条带光密度值之比(e/c)作数据分析,以其比值反映NHE-1mRNA的表达程度。应用Fluo3/Amy荧光探针标记,激光共聚焦观察细胞内钙离子的浓度。结果:在LPS(0.01～1.0μg/ml)作用48h后,MTT比色吸光度值分别为0.35±0.07,0.31±0.07,0.26±0.04,低于对照组0.44±0.06(P<0.01)。e/c分别为0.43±0.11,0.83±0.14,1.22±0.19,高于对照组0.28±0.08(P<0.01,n=4)。激光共聚焦结果显示LPS引起细胞内钙离子增多,呈剂量依赖型。结论:LPS可以引起肺动脉内皮细胞NHE-1表达增加,抑制细胞增殖,导致细胞死亡,钙离子可能参与NHE-1的激活及表达。
Abstract:
AIM To study the changes of sodium-hydrogen exchanger isoform-1(NHE-1)on bovine pulmonary artery endothelial cells with the function of lipopolysaccharide(LPS),and the relationship with endothelial cells death.METHODS:Endothelial Cells were separated from bovine pulmonary artery and cultured in vitro.Cell activities were reflected by the OD value ofMTT. Total RNA extraction and RT-PCR method were used to measure the mRNA expression of NHE-1. Intracellular free Ca2+ concentration was measured by calcium fluorescent probe Fluo3/AM and laser confocal microscope.RESULTS: In the presence of LPS(0.01～1.0μg/ ml), after 48 hours, the absorbencies of MTT (0.35±0.07,0.31±0.07,0.26±0.04)were lower in the experimental group than in the control group(0.44±0.06)(P<0.01),bute/c(0.43±0.11,0.83±0.14,1.22±0.19)were higher than control group(0.28±0.08)(P<0.01,n=4). Fluoresent intensity of intracellular free Ca2+ increased in a dose-dependant manner after the addition of LPS.CONCLUSION:LPS can cause a increased expression of NHE-1 mRNA,which may play an important role in endothelial cells death and intracellular free Ca2+ may participate in the activition and expression of NHE-1

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备注/Memo

备注/Memo:
收稿日期：2002-10-18.
更新日期/Last Update: 2003-05-01