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|本期目录/Table of Contents|

К阿片受体介导的抗心律失常作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2004年第2期
页码:
109-112
栏目:
基础研究
出版日期:
2004-03-01

文章信息/Info

Title:
Antiarrhythmic effect of К-opioid receptor stimulation in rats
作者:
姚青1; 王跃民2; 张鹏2; 毕辉2; 裴建明2
第四军医大学: 1.西京医院血管内分泌外科,2.生理学教研室,陕西 西安,710032
Author(s):
YAO Qing1; WANG Yue-min2; ZHANG Peng2; BI Hui2; PEI Jian-ming2
1.Department of Vascular Endocrine Surgery, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, China
关键词:
К阿片受体; 心肌缺血; 心律失常
Keywords:
К-opioidreceptor; myocardialischemia; arrhythmia
分类号:
R331.3
DOI:
-
文献标识码:
A
摘要:
目的:研究激动К阿片受体对大鼠心肌缺血性心律失常的影响。方法:在结扎大鼠冠状动脉的同时,静脉注射选择性К阿片受体激动剂U50488H,观察其对大鼠心率(HR)、左心室内压(LVSP)和室性心律失常发生的影响;分离大鼠心室肌细胞,观察U50488H对细胞内钙和L-型钙电流的影响。结果:心肌缺血后,大鼠HR无明显变化,LVSP显著降低,心电图显示了缺血性改变和心律失常发生;心肌缺血并给予U50488H后,HR显著减慢,LVSP进一步降低,大鼠心肌缺血性心律失常的发生率以及心律失常评分明显降低。U50488H可明显降低心肌细胞的钙瞬变,并能显著降低L-型钙电流。50488H的作用均可被选择性К阿片受体阻断剂nor-BNI阻断。结论:激动К阿片受体可减少大鼠心肌缺血性心律失常的发生,其机制可能与其抑制L-型钙通道和细胞内钙有关。
Abstract:
AIM:To investigate the effects of К-opioid receptor stimulation on myocardial ischemic arrhythmias in rats. METHODS: The coronary artery was ligated and simultaneously with U50488H, aselective К-opioid receptor agonist, was administered in travenously. Heart rate (HR), left ventricular systolic pressure (LVSP) and the incidence of ventricular arrhythmia were examined in rats. The effect of U50488H on intracellular Ca2+ and L-type Ca2+ channel current was also determined in the isolated ventricular myocytes. RESULTS: Following myocardial ischemia, HR did not change, LVSP decreased significantly and ECG showed typical ischemic changes and occurrences of different ventricular arrythmias. With the administration ofU50488H, the incidence of ventricular arrhythmia and arrhythmia score were significantly lowered. Further study showed that the intracellular Ca2+ transient and L-type Ca2+ channel current were inhibited by U50488H. The effects of U50488H were abolished in the presence of nor-BNI, aselective К-opioid receptor antagonist. CONCLUSION: К-opioid receptor stimulation decreases the incidence of ventricular ischemic arrhythmias, and th emechanism involves theinhibition of the intracellular Ca2+ and L-type Ca2+ channel.

参考文献/References

[1]Tai KK,Jin WQ,Chan TYK,et al.Characterization of [3H]-U69593 binding sites in the rat heart by recepto rbinding studies[J]. J Mol Cell Cardiol, 1991,23:1297-1302.

[2]Wang GY,Wu S,Pei JM,et al. К- but not δ-opioid receptor mediate effects of ischemic preconditioning on both infarct and arrhythmia in rats[J]. Am J Physiol Heart Circ Physiol, 2001,280:H384-H391.

[3]Uchida S,Suzuki A,Hotta H,et al.Cardiac actions of opioids[J].Neurosci Lett,1999,269:161-164.

[4]Schultz JJ,Hsu AK,Gross GJ.Ischemic preconditioning in theintac trat heart i smediated by d1 but not mork-opioi dreceptors [J]. Circulation, 1998,97:1282-1289.

[5]裴建明,毕辉,朱妙章.阿片样物质与心脏缺血预处理[J].生理科学进展,2003,34(1):63-66.

[6]Pugsley MK,Penz WP,Walker MJ,et al.Antiarrhythmic effects of U50, 488 Hin rats subject to coronary artery occlusion [J].Eur J Pharmacol,1992,212:15-19.

[7]Pei JM,Kravtsov GM,Wu S,et al. Calcium homeostasis in rat cardiomyocytes during chronic hypoxia: a time course study[J].Am J Physiol Cell Physiol, 2003,285(6): C1420-C1428.

[8]Chen M,Li LS,Wang YM,e tal.Negative effects of К-opioid receptor and its modulatory effects to β-ad renoceptor on heart[J].Di-SiJunyi Daxue Xuebao(J Fourth Mil Med Univ),2001,22(10):885-887.

[9]Yu XC,Wang HX,Pei JM.Anti-arrhythmic effect of К-opioid receptor stimulation in the perfused rat heart: Involvement of a camp-dependent pathway[J]. J Mol Cell cardiol, 1999,31:1809-1819.

[10]Coles JJA,Sigg DC,Iaizzo PA.The role of kappa-opioid receptor activation in pharmacological preconditioning of swine[J].Am J Physiol Heart Circ Physiol,2003,284(6):H2091-H2099.

[11]陈迈,贾国良,裴建明,等.U50488H预处理大鼠诱导的延迟性心脏保护效应及其机制[J].心脏杂志,2003,15(5):397-399.

备注/Memo

备注/Memo:
收稿日期：2003-3-4
更新日期/Last Update: 2004-03-01