不同方法建立动脉粥样硬化大鼠模型的比较(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2005年第1期
- 页码:
- 18-20
- 栏目:
- 基础研究
- 出版日期:
- 2005-01-05
文章信息/Info
- Title:
- Comparison of various atherosclerotic rat models
- Author(s):
- SHEN Li1; LU Weisheng1; YAO Junyu2; FANG Genqiang1; WANG Yichen1
- 1. Department of geriatrics, Xinhua Hospital, SSMU, Shanghai 200092, China
- Keywords:
- model; atherosclerosis; rat; pathology
- 分类号:
- R541.4
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 比较3种不同方法建立的动物模型，探索建立造型简便且重复性好的大鼠动脉粥样硬化模型。 方法 健康雄性SD大鼠（体质量150~170 g）24只，按3种方法建立模型： ① 高脂造模组，高脂饲料9周；② 维生素D3造模组，维生素D3 40万单位灌胃3 d，标准饲料9周；③ 高脂维生素D3造模组,维生素D3 20万单位灌胃3 d，高脂饲料9周。采用HE染色鉴定各组的动脉粥样硬化病变形成情况。 结果 光镜下高脂组血管各层结构基本正常；维生素D3造模组出现典型动脉粥样病变的斑块结构；高脂维生素D3造模组血管壁钙化和平滑肌细胞增生，程度较维生素D3组减轻，未出现典型斑块结构。 结论 40万单位维生素D3 的造模方法能在较短时间内建立典型成熟的动脉粥样硬化斑块模型。
- Abstract:
- AIM To investigate the simple and successful method for establishing atherosclerotic rat model . METHODS Ｔhree groups of rats were obtained: high-fat fed (６ SD rats with high-fat diet for ９ weeks) ；vitamin D-treated (9 SD rats treated by 400,000 unit vitamin D for three days, common diet for ９ weeks )；vitamin Dtreated and highfat fed (9 SD rats fed by 200,000 unit vitamin D for 3 days and highfat diet for ９ weeks)。HE staining was used to verify the atherosclerotic lesion of three models. RESULTS high-fat fed rats almost had no morphologic change in light microscope, while vitamin D-treated rats existd typical atherosclerosis. Vitamin Dtreated and high-fat fed rats also showed semblable but much milder pathologic changes in light microscope . CONCLUSION Method of 400,000 unit vitamin D for three days, common diet for 9 weeks is convenient and stable to establish typical atherosclerotic rat model .
参考文献/References
［1］ 郭延松，杨军珂. 鼠动脉粥样硬化模型的建立［J］.中国动脉硬化杂志，2003，11（1）：84-85.
［2］FleckensteinGrun G, Frey M, Luley C, et al. Differentiation calcium and cholesteroldominated types of atherosclerosis lesion: antiarteriosclerotic aspects of calcium antagonist［J］. Cardiovas Pharmacol, 1991，18（ suppl 6）:s1-s9.
［3］Fleckenstein KN, Kehel L, Bouayadi FE, et al. New model of atherosclerosis in insulin resistant sand rats: hypercholesterolemia combined with D2 vitamin［J］. Atherosclerosis ，2000，150:55-61.
［4］Price PA, Faus SA, Williamson MK. Warfarin-induced artery calcification is accelerated by growth and vitamin D［J］. Atherosclerosis Thromb Vasc Biol ，2000，20:317-323.
［5］温进坤，韩梅，杜玮南，等. 一种快速建立大鼠动脉粥样硬化模型的方法［J］.中国老年学杂志，2001，21：50-52.
［6］Price PA, June HH, Buckley JR, et al. Osteoprotegerin inhibits artery calcigfication induced by warfarin and by vitamin D［J］. Arterioscler Thromb Vasc Biol，2001，21:1610-1616.
［7］孙安阳，俞彰，钟慈声，等. 大鼠动脉钙超负荷模型的建立及确证［J］.中华医学杂志，1999，79（10）：769-772.
［8］Bennani KN, Kehel L, Bouayadi F, et al. New model of atherosclerosis in sand rats subjected to a high cholesterol diet and vitamin D2［J］. Therapie，1999，54: 559-565.
［9］Waston KE. Pathophysiology of coronary calcification［J］. J Cardiovasc Risk，2000，7:93-97.
备注/Memo
- 备注/Memo:
- 收稿日期：2004-07-27.
常用功能
统计/Statistics
- 摘要浏览/Viewed1117
- 全文下载/Downloads687
- 评论/Comments
